1008363-58-3Relevant articles and documents
Assessing the intracellular fate of rhodium(II) complexes
Minus, Matthew B.,Kang, Marci K.,Knudsen, Sarah E.,Liu, Wei,Krueger, Michael J.,Smith, Morgen L.,Redell, Michele S.,Ball, Zachary T.
, p. 11685 - 11688 (2016)
Rhodium(ii)-fluorophore conjugates have strong rhodium-based fluorescence quenching that can be harnessed to report on a conjugate's cellular uptake and the intracellular decomposition rate. Information gleened from this study allowed the design of an improved STAT3 metalloinhibitor.
Bisquinolinium-fluorescein conjugates as specific fluorescence probes of c-myc Pu22 G-quadruplex and their bioimaging and anticancer activities
Li, Jun-Hui,Ma, Tian-Zhu,Fu, Jia-Luo,Huang, Jun-Tao,Zhang, Meng-Jia,You, Pei-Dan,Zhou, Chun-Qiong
, (2021)
Targeting G-quadruplexes in the c-myc gene promoter region is one of the few therapeutic opportunities through inhibiting the c-myc protein overexpression. However, the design of probes recognizing c-myc G-quadruplexes with high selectivity and specificity and the more evaluation of cellular system still remain a big challenge. Here, two novel bisquinolinium-fluorescein conjugates (1a and 1b) with the alkyl linkers, have been designed and evaluated their selectivity and specificity for parallel c-myc Pu22 G-quadruplex. Compared with conjugate 1b with longer alkyl linker, conjugate 1a with shorter alkyl linker, exhibited higher fluorescence response and specificity towards c-myc Pu22 G-quadruplex than other wild-type c-myc G-quadruplexes, human telomere G-quadruplexes, other G-quadruplexes in the promoter regions and double-stranded DNA. According to the binding mode, the interaction of compound 1a with the 2nd loop around the 3′-end G-quartet through regulating the length of the alkyl linker, excited its fluorescence and enhanced its selectivity towards c-myc Pu22 G-quadruplex. Furthermore, conjugate 1a could selectively recognize c-myc Pu22 G-quadruplex DNA in cells through microscopy experiments, and inhibit cell proliferation possibly by reducing c-myc protein expression in cancer cells. This study provides guidance to design the high-performance fluorescence probes towards c-myc G-quadrulex by regulating the alkyl linker in the conjugate of G-quadruplex binder and fluorescence ligand, and develop anticancer drugs targeting c-myc G-quadruplex.
Self-assembly of magnetically recoverable ratiometric Cu2+ fluorescent sensor and adsorbent
Lu, Deli,Teng, Fei,Liu, Yunchang,Lu, Liujia,Chen, Chen,Lei, Juying,Wang, Lingzhi,Zhang, Jinlong
, p. 18660 - 18667 (2014)
A mesoporous shell containing a fluorescence resonance energy transfer (FRET) type of ratiometric Cu2+ fluorescent sensor was coated around a cetyltrimethylammonium bromide (CTAB) stabilized Fe3O 4@SiO2 core. All of the units comprising the sensor composite, including the Cu2+ ligand, signal reference and reporter units, are independent of each other and without a direct covalent linkage between them, and are site-selectively self-assembled into the pore framework or channel of the mesoporous silica matrix through the electrostatic interaction between CTAB porogen and silicates. The coordination of Cu2+ and its ligand leads to the variation of fluorescence energy transfer efficiency between neighboring FRET pairs benefiting from the nanosized pore system of the mesoporous matrix, which finally results in the ratiometric detection of Cu 2+. Investigation of Cu2+ adsorption performance indicated rapid removal efficiency, with a maximum adsorption capacity of 17.86 mg g -1. Fe3O4 nanoparticles were introduced as a core to make the sensor system magnetically recyclable after sensing and adsorption. Finally, the disassembly and reassembly of the Cu2+ sensor were achieved by extracting and reintroducing the units in CTAB micelles, making the sensing system reproducible.
