70672-06-9

Upstream product

• 67-56-1 methanol 
• 2321-07-5 fluorescein 
• 77-78-1 dimethyl sulfate 
• 74-88-4 methyl iodide 
• 1624845-20-0 Methyl 2-(3-oxo-6-((4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl)oxy)-3Hxanthen-9-yl)benzoate 
• 153120-93-5 5-amino-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid methyl ester 
• 518-45-6 fluorescein free acid 

Downstream Products

• 188584-20-5 2-(3-Oxo-6-undec-10-ynyloxy-3H-xanthen-9-yl)-benzoic acid methyl ester 
• 53677-99-9 2-(6'-methoxy-3'-oxo-3'H-xanthene-9'-yl)-benzoic acid methyl ester 
• 934016-26-9 2-[6-(11-hydroxyundecyloxy)-3-oxo-3H-xanthen-9-yl]benzoic acid methyl ester 
• 1227413-23-1 C₂₉H₂₈O₇ 
• 518-45-6 fluorescein free acid 
• 1395879-33-0 fluorescein methyl ester mono(β-D-galactopyranoside) 
• 1395879-36-3 fluorescein methyl ester mono(β-D-glucopyranoside) 
• 1395879-32-9 fluorescein methyl ester mono(2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside) 
• 1395879-35-2 fluorescein methyl ester mono(2,3,4,6-tetra-O-acetyl-β-D-glucopyranoside) 

Relevant academic research and scientific papers

A fluorescein-based fluorescence probe for the fast detection of thiol

A new turn-on fluorescent probe for the selective detection of thiol over other amino acids was synthesized. Probe possesses the widely-used thiol-selective 2,4-dinitrobenzenesulfonyl (DNBS) group which can react with thiol and release the fluorescein whi

A fluoresceinophane: 19,4,16-trioxa-1(2,10)-anthracena-2(1,2)- benzenacyclohexadeca-phane-17,3(19 H)-dione

A novel macrocycle containing fluorescein, the highly fluorescent title compound, C31H32O5, has a xanthene core and a benzyl unit that are planar. The latter is rotated by 72.99 (3)° from the xanthene mean plane. The C11 alkyl tether and the xanthene grou

A highly selective visual detection of tabun mimic diethyl cyanophosphate (DCNP): Effective discrimination of DCNP from other nerve agent mimics

A new tabun specific visual detection protocol is reported. The chemodosimeter fluorescein-hydroxamate aldehyde undergoes tandem nucleophilic substitution followed by cyanohydrin reaction with DCNP and shows a specific spectroscopic behaviour. The probe molecule clearly distinguishes tabun mimic DCNP over other nerve agent simulants as well as strong electrophiles.

Purification of tetracysteine-tagged proteins by affinity. Chromatography using a non-fluorescent, photochemically stable bisarsenical affinity ligand

The use of genetically encoded small peptide tags such as polyhistidine and tetracysteine tags has become important for protein purification and enrichment. An improved affinity purification of tetracysteine (CCXXCC) tagged proteins has been achieved using a nonfluorescent, photochemically stable bisarsenical affinity ligand SplAsH. The photochemical stability of the SplAsHbiotin, shown in compound 5, is superior to FlAsH-EDT2 and ReAsH-EDT2. An application of the SplAsH tag for affinity purification of tetracysteine-tagged proteins is reported.

Synthesis and Studies of Fluorescein Based Derivatives for their Optical Properties, Urease Inhibition and Molecular Docking

Herein, we design and synthesized new fluorescein based derivatives by insitu formation of fluorescein ester and further treated with corresponding hydrazide and amine to yield respective compounds i.e. FB1, FB2, FB3 and FB4. The spectral purity and chara

Synthesis and conformation of a novel fluorescein-Zn-porphyrin dyad and intramolecular energy transfer

This study describes the synthesis and characterization of a new zinc porphyrin-fluorescein dyad, the two chromophoric units being covalently linked by a 1,2,3-triazole bridge. The latter was formed by Cu-catalyzed Huisgen 1,3-dipolar cycloaddition. The c

Endoplasmic Reticulum Targeting Reactive Oxygen Species Sensor Based on Dihydrofluorescein: Application of Endoplasmic Reticulum Stress

Endoplasmic reticulum (ER) has a unique redox environment, which plays critical roles in the organelle's function and in its pathological responses such as ER stress. In this work, we introduce an ER-targeting fluorogenic reactive oxygen species (ROS) chemosensor (ER-Flu) from copper(I)-catalyzed alkyne-azide cycloaddition of 3-propargyl ester of 2′,7′-dichlorodihydrofluorescein diacetate and N3-glibenclamide, which were adopted as a fluorogenic ROS sensing module and an ER-targeting module, respectively. Thereby, a series of confocal microscopic experiments of ER-Flu demonstrated that the sensor localizes in ER of the live cells and that ROS are elevated in the cells by ER stress inducers such as thapsigargin, brefeldin A, and tunicamycin.

A Single Enzyme Mediates the "quasi-Living" Formation of Multiblock Copolymers with a Broad Biomedical Potential

This study describes a unique "quasi-living"block copolymerization method based on an initiation by a single enzyme. We use this term to describe a process where a preformed polymer chain can be reactivated to continue propagating with a second or third comonomer without addition of new catalyst. The presented strategy involves a laccase (oxidoreductase) mediated initial polymerization of 4-hydroxyphenylacetic acid to a homopolymer containing phenolic terminal units, which in turn can be easily reactivated by the same enzyme in the same reaction vessel to continue propagation with a second monomer (tyramine). Increased copolymer yield (up to 26.0percent) and polymer molecular mass (up to Mw = 116 ?000 Da) are achieved through the addition of previously developed micellar and hydrogel enzyme complexing agents. The produced poly(tyramine)-b-poly(4-hydroxyphenylacetic acid)-b-poly(tyramine) is water-soluble and able to self-assemble in aqueous solution. Both tyramine blocks were successfully modified with ibuprofen moieties (up to 24.6percent w/w load) as an example for potential polymer drug conjugation. The copolymerization could be further extended with addition of a third (fluorescent) comonomer in the same reaction vessel to yield a fluorescent pentablock copolymer. The successful modifications and advantageous solution behavior of the produced copolymers demonstrate their viability as versatile drug delivery and/or bioimaging agents, as confirmed by cytotoxicity and cellular uptake studies.

Cholesteric fluorescent liquid crystal polysiloxanes with different liquid crystal side chain lengths—Synthesis and properties

With polymethylhydrogensiloxane (PMHS) as the main chain, two series side-chain cholesteric liquid crystal polymers (ChLCPs) with different liquid crystal side chain lengths were prepared by hydrosilylation reaction. Then two series of cholesteric fluorescent liquid crystal polymers (FL-ChLCPs) were obtained by etherification reaction. The obtained FL-ChLCPs have both excellent mesomorphic behavior, and great photoluminescent property. We confirmed their chemical structures by FT-IR, and 1H NMR spectroscopy, and found them with excellent thermal stability by TGA. We characterized their mesomorphic behavior and thermal performance by POM, DSC and XRD, and confirmed all the polymers belonged to cholesteric phase. FL-ChLCPs emitted different-intensity fluorescence under UV light, and we found some regularity by PL spectrum.

Detection reagent for fluorescently labeling amino compounds and application of detection reagent in protein fluorescence labeling

The invention belongs to the field of protein fluorescence labeling, and particularly relates to a detection reagent for fluorescently labeling amino compounds and application of the detection reagentin protein fluorescence labeling. The detection reagent is shown as a structural general formula (1), and in the general formula (1), R represents-OH, -OCH3, -OCH2CH3, -OCH(CH3)2 and -OC(CH3)3. The detection reagent provided by the invention has the advantages of good marking product stability, high marking efficiency, high fluorescence quantum yield, short marking time and simple reaction conditions during marking.