70672-06-9Relevant articles and documents
A fluorescein-based fluorescence probe for the fast detection of thiol
Liu, Yunchang,Xiang, Kaiqiang,Tian, Baozhu,Zhang, Jinlong
, p. 2478 - 2483 (2016)
A new turn-on fluorescent probe for the selective detection of thiol over other amino acids was synthesized. Probe possesses the widely-used thiol-selective 2,4-dinitrobenzenesulfonyl (DNBS) group which can react with thiol and release the fluorescein whi
A fluoresceinophane: 19,4,16-trioxa-1(2,10)-anthracena-2(1,2)- benzenacyclohexadeca-phane-17,3(19 H)-dione
Dufresne, Stephane,Perez Guarn, Sergio Andres,Skene
, p. o350-o352 (2007)
A novel macrocycle containing fluorescein, the highly fluorescent title compound, C31H32O5, has a xanthene core and a benzyl unit that are planar. The latter is rotated by 72.99 (3)° from the xanthene mean plane. The C11 alkyl tether and the xanthene grou
A highly selective visual detection of tabun mimic diethyl cyanophosphate (DCNP): Effective discrimination of DCNP from other nerve agent mimics
Goud, D. Raghavender,Pardasani, Deepak,Tak, Vijay,Dubey, Devendra Kumar
, p. 24645 - 24648 (2014)
A new tabun specific visual detection protocol is reported. The chemodosimeter fluorescein-hydroxamate aldehyde undergoes tandem nucleophilic substitution followed by cyanohydrin reaction with DCNP and shows a specific spectroscopic behaviour. The probe molecule clearly distinguishes tabun mimic DCNP over other nerve agent simulants as well as strong electrophiles.
Purification of tetracysteine-tagged proteins by affinity. Chromatography using a non-fluorescent, photochemically stable bisarsenical affinity ligand
Ying, Lai-Qiang,Branchaud, Bruce P.
, p. 987 - 992 (2011)
The use of genetically encoded small peptide tags such as polyhistidine and tetracysteine tags has become important for protein purification and enrichment. An improved affinity purification of tetracysteine (CCXXCC) tagged proteins has been achieved using a nonfluorescent, photochemically stable bisarsenical affinity ligand SplAsH. The photochemical stability of the SplAsHbiotin, shown in compound 5, is superior to FlAsH-EDT2 and ReAsH-EDT2. An application of the SplAsH tag for affinity purification of tetracysteine-tagged proteins is reported.
Synthesis and Studies of Fluorescein Based Derivatives for their Optical Properties, Urease Inhibition and Molecular Docking
Mahajan, Prasad G.,Dige, Nilam C.,Vanjare, Balasaheb D.,Raza, Hussain,Hassan, Mubashir,Seo, Sung-Yum,Hong, Seong-Karp,Lee, Ki Hwan
, p. 1305 - 1315 (2018)
Herein, we design and synthesized new fluorescein based derivatives by insitu formation of fluorescein ester and further treated with corresponding hydrazide and amine to yield respective compounds i.e. FB1, FB2, FB3 and FB4. The spectral purity and chara
Synthesis and conformation of a novel fluorescein-Zn-porphyrin dyad and intramolecular energy transfer
Rezazgui, Olivier,Trouillas, Patrick,Qiu, Shi-Hong,Siegler, Benjamin,Gierschner, Johannes,Leroy-Lhez, Stephanie
, p. 3843 - 3856 (2016)
This study describes the synthesis and characterization of a new zinc porphyrin-fluorescein dyad, the two chromophoric units being covalently linked by a 1,2,3-triazole bridge. The latter was formed by Cu-catalyzed Huisgen 1,3-dipolar cycloaddition. The c
Endoplasmic Reticulum Targeting Reactive Oxygen Species Sensor Based on Dihydrofluorescein: Application of Endoplasmic Reticulum Stress
Le, Hoa Thi,Jo, Hye-Ryeong,Oh, Se-Yun,Jung, Jinwook,Kim, Young Gi,Kang, Chulhun,Kim, Tae Woo
supporting information, p. 279 - 285 (2020/12/21)
Endoplasmic reticulum (ER) has a unique redox environment, which plays critical roles in the organelle's function and in its pathological responses such as ER stress. In this work, we introduce an ER-targeting fluorogenic reactive oxygen species (ROS) chemosensor (ER-Flu) from copper(I)-catalyzed alkyne-azide cycloaddition of 3-propargyl ester of 2′,7′-dichlorodihydrofluorescein diacetate and N3-glibenclamide, which were adopted as a fluorogenic ROS sensing module and an ER-targeting module, respectively. Thereby, a series of confocal microscopic experiments of ER-Flu demonstrated that the sensor localizes in ER of the live cells and that ROS are elevated in the cells by ER stress inducers such as thapsigargin, brefeldin A, and tunicamycin.
Detection reagent for fluorescently labeling amino compounds and application of detection reagent in protein fluorescence labeling
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Paragraph 0038-0045, (2020/12/09)
The invention belongs to the field of protein fluorescence labeling, and particularly relates to a detection reagent for fluorescently labeling amino compounds and application of the detection reagentin protein fluorescence labeling. The detection reagent is shown as a structural general formula (1), and in the general formula (1), R represents-OH, -OCH3, -OCH2CH3, -OCH(CH3)2 and -OC(CH3)3. The detection reagent provided by the invention has the advantages of good marking product stability, high marking efficiency, high fluorescence quantum yield, short marking time and simple reaction conditions during marking.
Novel fluorescein derivative compound, process for the preparation thereof and method for detecting zinc (Zn2+) ion using the same
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Paragraph 0052-0055, (2020/07/01)
The present invention provides a method for detecting zinc (Zn^2+) ions by synthesizing N-(3andprime;,6andprime;-dihydroxy-3-oxospyro[isoindoline-1,9andprime;-xanthene]-2-yl)-2-hydroxybenzamide, followed by using fluorescent organic nanoparticles obtained through a reprecipitation method. A novel fluorescein derivative compound, a manufacturing method thereof, and a method for detecting zinc ions using the same of the present invention exhibit a very low detection limit (0.0011 andmu;g/ml), are non-toxic, and can detect zinc ions in living cells, thereby being able to be usefully used in biomedical and environmental fields.COPYRIGHT KIPO 2020
A Single Enzyme Mediates the "quasi-Living" Formation of Multiblock Copolymers with a Broad Biomedical Potential
Gitsov, Ivan,Guo, Dandan,Luo, Juntao,Scheibel, Dieter Michael
, p. 2132 - 2146 (2020/07/15)
This study describes a unique "quasi-living"block copolymerization method based on an initiation by a single enzyme. We use this term to describe a process where a preformed polymer chain can be reactivated to continue propagating with a second or third comonomer without addition of new catalyst. The presented strategy involves a laccase (oxidoreductase) mediated initial polymerization of 4-hydroxyphenylacetic acid to a homopolymer containing phenolic terminal units, which in turn can be easily reactivated by the same enzyme in the same reaction vessel to continue propagation with a second monomer (tyramine). Increased copolymer yield (up to 26.0percent) and polymer molecular mass (up to Mw = 116 ?000 Da) are achieved through the addition of previously developed micellar and hydrogel enzyme complexing agents. The produced poly(tyramine)-b-poly(4-hydroxyphenylacetic acid)-b-poly(tyramine) is water-soluble and able to self-assemble in aqueous solution. Both tyramine blocks were successfully modified with ibuprofen moieties (up to 24.6percent w/w load) as an example for potential polymer drug conjugation. The copolymerization could be further extended with addition of a third (fluorescent) comonomer in the same reaction vessel to yield a fluorescent pentablock copolymer. The successful modifications and advantageous solution behavior of the produced copolymers demonstrate their viability as versatile drug delivery and/or bioimaging agents, as confirmed by cytotoxicity and cellular uptake studies.
