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Usage

Uses

Used in Medical Research:
4-Methyl-2-oxo-2H-1-benzopyran-7-yl-β-D-glucopyranosiduronic Acid Methyl Ester 2,3,4-Triacetate is used as a fluorogenic substrate for the detection and measurement of α-L-Iduronidase enzyme activity. This is crucial in the diagnosis and monitoring of Hurler's syndrome, as the enzyme deficiency is a key indicator of the disease.
Used in Diagnostic Applications:
In the field of diagnostics, 4-Methyl-2-oxo-2H-1-benzopyran-7-yl-β-D-glucopyranosiduronic Acid Methyl Ester 2,3,4-Triacetate serves as a valuable tool for identifying individuals with Hurler's syndrome. By assessing α-L-Iduronidase enzyme activity, medical professionals can determine the presence of the syndrome and initiate appropriate treatment plans.
Used in Pharmaceutical Development:
4-Methyl-2-oxo-2H-1-benzopyran-7-yl-β-D-glucopyranosiduronic Acid Methyl Ester 2,3,4-Triacetate is also utilized in the development of pharmaceuticals targeting Hurler's syndrome. Researchers can use 4-Methyl-2-oxo-2H-1-benzopyran-7-yl-β-D-glucopyranosiduronic Acid Methyl Ester 2,3,4-Triacetate to study the enzyme's function and develop potential treatments to address the enzyme deficiency, thereby improving the quality of life for individuals affected by the syndrome.

Upstream product

• 90-33-5 7-hydroxy-4-methyl-chromen-2-one 
• 7355-18-2 (2S,3S,4S,5R,6S)-3,4,5,6-Tetraacetoxy-tetrahydro-pyran-2-carboxylic acid methyl ester 

Downstream Products

• 116523-82-1 methyl 4-methylcoumarin-7-yl-β-D-glucopyranuronate 
• 6160-80-1 4-methylumbelliferyl-β-D-glucuronide 
• 128095-50-1 4-methylcoumarin-7-yl-2,3,4-tri-O-acetyl-α-L-iduronic acid methyl ester 
• 66966-09-4 4-methylcoumarin-7-yl α-L-idopyranosiduronic acid 
• 128095-52-3 4-methylcoumarin-7-yl-α-L-iduronic acid methyl ester 

Relevant academic research and scientific papers

An improved helferich method for the α/β-stereoselective synthesis of 4-methylumbelliferyl glycosides for the detection of microorganisms

An improved Helferich method is presented. It involves the glycosylation of 4-methyl-umbelliferone with glycosyl acetates in the presence of boron trifluoride etherate combined with triethylamine, pyridine, or 4-dimethylaminopyridine under mild conditions, followed by deprotection to give fluorogenic 4-methylumbelliferyl glycoside substrates. Due to the use of base, the glycosylation reaction proceeds more easily, is uncommonly α- or β-stereoselective, and affords the corresponding products in moderate to excellent yields (51%-94%) under appropriate conditions.