1023637-24-2Relevant academic research and scientific papers
Synthesis of a common tetrasaccharide motif of Haemophilus influenzae LPS inner core structures
Mannerstedt, Karin,Segerstedt, Eva,Olsson, Johan,Oscarson, Stefan
experimental part, p. 1087 - 1091 (2009/02/05)
A conserved tetrasaccharide structure, l-glycero-α-d-manno- heptopyranosyl-(1→2)-(6-O-aminoethylphosphono-l-glycero-α-d-manno- heptopyranosyl)-(1→3)-[β-d-glucopyranosyl-(1→4)] -l-glycero-α-d-manno-heptopyranose, from the LPS inner core of Haemophilus influenzae has been synthesised as its ethylamino glycosides to allow later conjugations. Starting from a previously synthesised suitably protected trisaccharide intermediate, the third heptose and subsequently the spacer were introduced using thioglycoside donor chemistry. The phosphoethanolamine was formed employing a Boc-protected phosphoamidite. Final deprotection and conjugation to biotin gave conjugates that will be used to study the specificity of MAbs raised against native LPS structures.
