1042435-55-1Relevant articles and documents
N-Myristoyl transferase-mediated protein labelling in vivo
Heal, William P.,Wickramasinghe, Sasala R.,Leatherbarrow, Robin J.,Tate, Edward W.
experimental part, p. 2308 - 2315 (2009/02/02)
N-Myristoyl transferase-mediated labelling using a substrate modified with an azide or alkyne tag is described as an efficient and site-selective method for the introduction of a bioorthogonal tag at the N-terminus of a recombinant protein. The procedure may be performed in vitro, or in a single over-expression/tagging step in vivo in bacteria; tagged proteins may then be captured using Staudinger-Bertozzi or 'click' chemistry protocols to introduce a secondary label for downstream analysis. The straightforward synthesis of the chemical and molecular biological tools described should enable their use in a wide range of N-terminal labelling applications.
