106070-31-9

Usage

Uses

Used in Cell Biology and Medical Research:
2',7'-dichlorodihydrofluorescein is used as a fluorescent indicator for monitoring the presence and activity of reactive oxygen species (ROS) in living cells. Upon reaction with ROS, it is oxidized to form the highly fluorescent compound 2',7'-dichlorofluorescein, which can be visualized and measured using fluorescence microscopy or spectroscopy.
Used in Oxidative Stress and Inflammation Studies:
2',7'-dichlorodihydrofluorescein is employed as a valuable tool for studying oxidative stress and inflammation in biological systems. Its ability to detect ROS makes it instrumental in understanding the role of oxidative stress in various physiological and pathological processes.
Used in Disease Research:
2',7'-dichlorodihydrofluorescein has potential applications in studies of diseases characterized by oxidative stress and inflammation, such as cancer, diabetes, and neurodegenerative disorders. By monitoring ROS levels, researchers can gain insights into the mechanisms underlying these diseases and explore potential therapeutic interventions.

Upstream product

• 76-54-0 2',7'-dichlorofluorescein 
• 4091-99-0 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid 
• 18362-80-6 2,7-dichlorofluorescein 

Downstream Products

• 76-54-0 2',7'-dichlorofluorescein 
• 52450-38-1 N¹-acetyl-N²-formyl-5-methoxykynurenine 
• 861669-89-8 2-[3,6-bis-(2,2-dimethoxyethoxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid 2,2-dimethoxyethyl ester 
• 18362-80-6 2,7-dichlorofluorescein 

Relevant academic research and scientific papers

Pyrocatalytic oxidation-strong size-dependent poling effect on catalytic activity of pyroelectric BaTiO3nano- And microparticles

Pyrocatalysis is an emerging advanced oxidation process for wastewater remediation with the potential for thermal energy harvesting and utilization. Although several studies explored the potential of new pyrocatalyst materials to degrade harmful organic water pollutants, the role of important material properties and electric poling procedures on the pyrocatalytic activity is still unclear. In this work, we investigate the interdependence between particle size, electric poling and pyrocatalytic activity of BaTiO3 powders with nominal particle sizes of 100, 200 and 500 nm by using the dichlorofluorescein redox assay. Depending on the particle size, the influence of surface area or phase composition on the pyrocatalytic activity predominates. Moreover, we demonstrate that poling of pyrocatalysts leads to a strong size-dependent increase of pyrocatalytic activity. This poling effect increases with particle size up to +247% and can be explained with size-dependent changes in phase composition and domain structure. Combining all results, the progression of the pyrocatalytic activity as a function of particle size was derived and a future strategy for maximizing the catalytic performance of pyrocatalysts was developed. This study greatly improves the understanding about the role of important material properties and electric poling on pyrocatalytic activity, thus enabling an effective catalyst design. With the help of highly active catalysts, the pyrocatalytic process can take the next step in its development into a new and energy-efficient advanced oxidation process for water remediation.

Photoinduced Dynamics and Toxicity of a Cancer Drug in Proximity of Inorganic Nanoparticles under Visible Light

Drug sensitization with various inorganic nanoparticles (NPs) has proved to be a promising and an emergent concept in the field of nanomedicine. Rose bengal (RB), a notable photosensitizer, triggers the formation of reactive oxygen species under green-lig

Melatonin enhances photo-oxidation of 29, 79-dichlorodihydrofluorescein by an antioxidant reaction that renders N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK)

The indolamine melatonin (MEL) is described as an antioxidant and a free radical scavenger. However occasionally, the indoleamine has been reported to increase free radicals with insufficient mechanistic explanation. In an attempt to find a reason for tho

Photodamage in a mitochondrial membrane model modulated by the topology of cationic and anionic meso-tetrakis porphyrin free bases

The photodynamic effects of the cationic TMPyP (meso-tetrakis [N-methyl-4-pyridyl]porphyrin) and the anionic TPPS4 (meso-tetrakis[4- sulfonatophenyl]porphyrin) against PC/CL phosphatidylcholine/cardiolipin (85/15%) membranes were probed to address the inf

Active oxygen chemistry within the liposomal bilayer: Part IV: Locating 2′,7′-dichlorofluorescein (DCF), 2′,7′- dichlorodihydrofluorescein (DCFH) and 2′,7′- dichlorodihydrofluorescein diacetate (DCFH-DA) in the lipid bilayer

2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) is commonly used to detect the generation of reactive oxygen intermediates and for assessing the overall oxidative stress in toxicological phenomenon. It has been suggested that DCFH-DA crosses the cell

Novel one pot synthesis and spectroscopic characterization of a folate-Mn3O4 nanohybrid for potential photodynamic therapeutic application

Treatment of cancer using nanoparticles made of inorganic and metallic compounds has been increasingly used, owing to their novel intrinsic physical properties and their potential to interact with specific cellular sites, thereby significantly reducing se

Ultrasound-dependent cytoplasmic internalization of a peptide-sonosensitizer conjugate

A method to induce cytoplasmic peptide delivery, using ultrasound, was demonstrated using a molecular conjugate of a cell-penetrating peptide (CPP), a functional peptide, and a sonosensitizer. As a model of such molecular conjugates, TatBim-RB, consisting

Coordination-Accelerated Radical Formation from Acyclic Enediynes for Tumor Cell Suppression

A maleimide-based acyclic enediyne with salicylaldiminato substituents at the alkyne termini was synthesized, which was further chelated with three kinds of metal-ions, CuII, ZnII, and MgII, and form metalloenediynes. The cycloaromatization of this thermally inactive enediyne ligand was greatly accelerated through the coordination with metal ions. Specifically, the CuII-metalloenediyne showed an extremely low onset temperature of 55 °C and underwent spontaneous cycloaromatization at ambient temperature to produce free radicals, followed by generation of reactive oxygen species in the physiological environment. The metalloenediyne exhibited excellent DNA cleavage ability and high cytotoxicity towards HeLa cells, with half-maximal inhibitory concentration values comparable to many commercial antitumor agents. The combination of the electron-withdrawing effect of the maleimide moiety at the ene position and metal coordination at the yne termini provides a new inspiration for designing and synthesizing highly efficient enediyne antitumor agents.

Identification of ros produced by photodynamic activity of chlorophyll/cyclodextrin inclusion complexes

Photodynamic therapy (PDT) is a way of treating malignant tumors and hyperproliferative diseases. It is based on the use of photosensitizer, herein the chlorophyll a (chl a), and a light of an appropriate wavelength. The interaction of the photosensitizer (PS) with the light produces reactive oxygen species (ROS), powerful oxidizing agents, which cause critical damage to the tissue. To solubilize chl a in aqueous solution and to obtain it as monomer, we have used cyclodextrins, carriers which are able to interact with the pigment and form the inclusion complex. The aim of this study is to examine which types of ROS are formed by Chl a/cyclodextrin complexes in phosphate buffered solution and cell culture medium, using specific molecules, called primary acceptors, which react selectively with the reactive species. In fact the changes of the absorption and the emission spectra of these molecules after the illumination of the PS provide information on the specific ROS formation. The 1O2 formation has been tested using chemical methods based on the use of Uric Acid (UA), 9,10-diphenilanthracene (DPA) and Singlet oxygen sensor green (SOSG) and by direct detection of Singlet Oxygen ( 1O2) luminescence decay at 1270 nm. Moreover, 2,7-dichlorofluorescin and ferricytochrome c (Cyt Fe3+) have been used to detect the formation of hydrogen peroxide and superoxide radical anion, which reduces Fe3+ of the ferricytochrome to Fe2+, respectively. For the first time, photodynamic activity in vitro of natural Chlorophyll a (Chl a) has been investigated evidencing which types of ROS are formed. Chl a has been solubilized in aqueous solution by means of various cyclodextrins forming inclusion complexes. The ROS production has been carried out in the system using specific molecules, called primary acceptors, which react selectively with the reactive species.

A metal-ion-releasing probe for DNA detection by catalytic signal amplification

(Figure Presented) Seeing the light: Upon binding to a complementary nucleic acid, a metal-complex-DNA conjugate releases copper(II) ions that activate the precatalyst 1,10-phenanthroline (phen) and amplify the primary signal through catalytic production