4091-99-0 Usage
Description
DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 59,500 M?1cm?1).
Chemical Properties
white to yellow-beige or pale pink powder
Uses
Different sources of media describe the Uses of 4091-99-0 differently. You can refer to the following data:
1. Cell-permeable fluorogenic probe used to measure reactive oxygen species (ROS) within cells by detection of enzymatically formed hydrogen peroxide.
2. 2',7'-Dichlorodihydrofluorescein as a cell-permeable non-fluorescent probe. It is de-esterified intracellularly and turns to highly fluorescent 2’7’dichlorofluorescein upon oxidation.
3. 2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.
General Description
Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (?NO) and for the determination of the degree of overall oxidative stress.
Biochem/physiol Actions
Cell permeable: yes
Check Digit Verification of cas no
The CAS Registry Mumber 4091-99-0 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 4,0,9 and 1 respectively; the second part has 2 digits, 9 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 4091-99:
(6*4)+(5*0)+(4*9)+(3*1)+(2*9)+(1*9)=90
90 % 10 = 0
So 4091-99-0 is a valid CAS Registry Number.
4091-99-0Relevant articles and documents
Synthesis of high contrast fluorescein-diethers for rapid bench-top sensing of palladium
Kitley, Weston R.,Santa Maria, Peter J.,Cloyd, Ryan A.,Wysocki, Laura M.
, p. 8520 - 8523 (2015)
A new series of palladium sensors based on fluorescein bis(allyl ether) compounds has been designed. We utilized reduced fluoresceins as key synthetic intermediates. These probes exhibit negligible background fluorescence and rapid reaction with palladium, allowing a concentration of 100 ppt to be detected in minutes using a handheld UV lamp. This journal is
