109785-33-3Relevant academic research and scientific papers
Probing substrate promiscuity of amylosucrase from neisseria polysaccharea
Daude, David,Champion, Elise,Morel, Sandrine,Guieysse, David,Remaud-Simeon, Magali,Andre, Isabelle
, p. 2288 - 2295 (2013/08/23)
The amylosucrase from Neisseria polysaccharea (NpAS) naturally catalyzes the synthesis of a variety of products from sucrose and shows signs of plasticity of its active site. To explore further this promiscuity, the tolerance of amylosucrase towards different donor and acceptor substrates was investigated. The selection of alternate donor substrates was first made on the basis of preliminary molecular modeling studies. From 11 potential donors harboring selective derivatizations that were experimentally evaluated, only p-nitrophenyl-α-D-glucopyranoside was used by the wild-type enzyme, and this underlines the high specificity of the -1 subsite of NpAS for glucosyl donor substrates. The acceptor substrate promiscuity was further explored by screening 20 hydroxylated molecules, including D- and L-monosaccharides as well as polyols. With the exception of one compound, all were successfully glucosylated, and this showcases the tremendous plasticity of the +1 subsite of NpAS, which is responsible for acceptor recognition. The products obtained from the transglucosylation reactions of three selected acceptors were characterized, and they revealed original structures and enzyme enantiopreference, which were more particularly analyzed by insilico docking analyses.
4-O-α-D-GALACTOPYRANOSYL-D-GALACTOSE: EFFICIENT SYNTHETIC ROUTES FROM "POLYGALACTURONIC ACID"
Dahmen, Jan,Frejd, Torbjoern,Lave, Thomas,Lindh, Frank,Magnusson, Goeran,et al.
, p. 219 - 224 (2007/10/02)
Enzymic hydrolysis of "polygalacturonic acid" gave a mixture of oligomers which was fractionated by ion-exchange chromatography.The resulting di- and trisaccharides were treated, respectively, with methanol and ethylene oxide, and the resulting esters were reduced with sodium borohydride.Treatment of the products with acetic anhydride and sulfuric acid, followed by deacetylation, produced the title compound.
