109785-33-3

Basic information

• Product Name: α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→4)-α-D-sorbitol
• CAS NO: 109785-33-3
• Molecular Weight: 506.458
• Mol File: 109785-33-3.mol
• Article Data: 2

Chemical Properties

• CAS DataBase Reference: α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→4)-α-D-sorbitol(CAS DataBase Reference)
• NIST Chemistry Reference: α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→4)-α-D-sorbitol(109785-33-3)
• EPA Substance Registry System: α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→4)-α-D-sorbitol(109785-33-3)

Safety Data

• Hazardous Substances Data: 109785-33-3(Hazardous Substances Data)

Upstream product

• 40307-13-9 tri-galacturonic acid 
• 585-88-6 MALTITOL 
• 57-50-1 Sucrose 

Relevant articles and documents

Probing substrate promiscuity of amylosucrase from neisseria polysaccharea

The amylosucrase from Neisseria polysaccharea (NpAS) naturally catalyzes the synthesis of a variety of products from sucrose and shows signs of plasticity of its active site. To explore further this promiscuity, the tolerance of amylosucrase towards different donor and acceptor substrates was investigated. The selection of alternate donor substrates was first made on the basis of preliminary molecular modeling studies. From 11 potential donors harboring selective derivatizations that were experimentally evaluated, only p-nitrophenyl-α-D-glucopyranoside was used by the wild-type enzyme, and this underlines the high specificity of the -1 subsite of NpAS for glucosyl donor substrates. The acceptor substrate promiscuity was further explored by screening 20 hydroxylated molecules, including D- and L-monosaccharides as well as polyols. With the exception of one compound, all were successfully glucosylated, and this showcases the tremendous plasticity of the +1 subsite of NpAS, which is responsible for acceptor recognition. The products obtained from the transglucosylation reactions of three selected acceptors were characterized, and they revealed original structures and enzyme enantiopreference, which were more particularly analyzed by insilico docking analyses.