1101181-65-0Relevant academic research and scientific papers
Ultrasensitive Detection of Salmonella and Listeria monocytogenes by Small-Molecule Chemiluminescence Probes
Roth-Konforti, Michal,Green, Ori,Hupfeld, Mario,Fieseler, Lars,Heinrich, Nadine,Ihssen, Julian,Vorberg, Raffael,Wick, Lukas,Spitz, Urs,Shabat, Doron
, p. 10361 - 10367 (2019)
Detection of Salmonella and L. monocytogenes in food samples by current diagnostic methods requires relatively long time to results (2–6 days). Furthermore, the ability to perform environmental monitoring at the factory site for these pathogens is limited due to the need for laboratory facilities. Herein, we report new chemiluminescence probes for the ultrasensitive direct detection of viable pathogenic bacteria. The probes are composed of a bright phenoxy-dioxetane luminophore masked by triggering group, which is activated by a specific bacterial enzyme, and could detect their corresponding bacteria with an LOD value of about 600-fold lower than that of fluorescent probes. Moreover, we were able to detect a minimum of 10 Salmonella cells within 6 h incubation. The assay allows for bacterial enrichment and detection in one test tube without further sample preparation. We anticipate that this design strategy will be used to prepare analogous chemiluminescence probes for other enzymes relevant to specific bacteria detection and point-of-care diagnostics.
Bioreversible protection of nucleoside diphosphates
Jessen, Henning Jacob,Schulz, Tilmann,Balzarini, Jan,Meier, Chris
supporting information; experimental part, p. 8719 - 8722 (2009/05/15)
(Figure Presented) Going incognito: A new prodrug approach has been developed to facilitate the diffusion of highly polar polyphosphorylated nucleosides across cell membranes (see scheme). Inside the cell, the masking groups on the nucleoside diphosphate
