1157-60-4 Usage
Uses
Used in Pharmaceutical Research and Development:
[5-(2,4-dioxo-1H-pyrimidin-5-yl)-3,4-dihydroxy-oxolan-2-yl]methoxyphosphonic acid is used as a research tool for studying the mechanisms of nucleic acid synthesis and function. Its structural resemblance to natural nucleotides allows it to be employed in the development of new drugs that target specific biological pathways or processes.
Used in Biochemical Analysis:
In the field of biochemistry, [5-(2,4-dioxo-1H-pyrimidin-5-yl)-3,4-dihydroxy-oxolan-2-yl]methoxyphosphonic acid is used as a reagent or probe to investigate the interactions between nucleotides and various enzymes or proteins. This can help in understanding the roles of these molecules in cellular processes and disease mechanisms.
Used in Molecular Biology Applications:
[5-(2,4-dioxo-1H-pyrimidin-5-yl)-3,4-dihydroxy-oxolan-2-yl]methoxyphosphonic acid is used as a component in molecular biology techniques, such as the synthesis of modified nucleic acids or the development of novel gene editing tools. Its unique properties may enable the creation of new methodologies for genetic research and manipulation.
Used in Drug Development for Targeted Therapies:
Given its potential to interfere with nucleic acid synthesis or function, [5-(2,4-dioxo-1H-pyrimidin-5-yl)-3,4-dihydroxy-oxolan-2-yl]methoxyphosphonic acid is used as a lead compound in the development of targeted therapies for various diseases, including cancer and viral infections. Its ability to modulate specific biological pathways could lead to the creation of more effective and less toxic treatments.
Used in Diagnostics:
[5-(2,4-dioxo-1H-pyrimidin-5-yl)-3,4-dihydroxy-oxolan-2-yl]methoxyphosphonic acid may also be utilized in the development of diagnostic tools, such as probes for detecting specific nucleic acid sequences or markers associated with certain diseases. This could improve the accuracy and efficiency of disease detection and monitoring.
Check Digit Verification of cas no
The CAS Registry Mumber 1157-60-4 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,1,5 and 7 respectively; the second part has 2 digits, 6 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 1157-60:
(6*1)+(5*1)+(4*5)+(3*7)+(2*6)+(1*0)=64
64 % 10 = 4
So 1157-60-4 is a valid CAS Registry Number.
InChI:InChI=1/C9H13N2O9P/c12-5-4(2-19-21(16,17)18)20-7(6(5)13)3-1-10-9(15)11-8(3)14/h1,4-7,12-13H,2H2,(H2,16,17,18)(H2,10,11,14,15)/t4-,5-,6-,7+/m1/s1
1157-60-4Relevant academic research and scientific papers
Huang, Siyu,Mahanta, Nilkamal,Begley, Tadhg P.,Ealick, Steven E.
, p. 9245 - 9255 (2012)
Pseudouridine (ψ), the most abundant modification in RNA, is synthesized in situ using ψ synthase. Recently, a pathway for the degradation of ψ was described [Preumont, A., Snoussi, K., Stroobant, V., Collet, J. F., and Van Schaftingen, E. (2008) J. Biol. Chem. 283, 25238-25246]. In this pathway, ψ is first converted to ψ 5′-monophosphate (ψMP) by ψ kinase and then ψMP is degraded by ψMP glycosidase to uracil and ribose 5-phosphate. ψMP glycosidase is the first example of a mechanistically characterized enzyme that cleaves a C-C glycosidic bond. Here we report X-ray crystal structures of Escherichia coli ψMP glycosidase and a complex of the K166A mutant with ψMP. We also report the structures of a ring-opened ribose 5-phosphate adduct and a ring-opened ribose ψMP adduct. These structures provide four snapshots along the reaction coordinate. The structural studies suggested that the reaction utilizes a Lys166 adduct during catalysis. Biochemical and mass spectrometry data further confirmed the existence of a lysine adduct. We used site-directed mutagenesis combined with kinetic analysis to identify roles for specific active site residues. Together, these data suggest that ψMP glycosidase catalyzes the cleavage of the C-C glycosidic bond through a novel ribose ring-opening mechanism.
