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Upstream product

• 28320-31-2 2-bromo-9,9-dimethyl-9H-fluorene 
• 28320-33-4 2-bromo-7-nitro-9,9-dimethyl-9H-fluorene 
• 4569-45-3 9,9-dimethyl-9H-fluorene 
• 28320-32-3 2,7-dibromo-9,9-dimethyl-9H-fluorene 
• 109-89-7 diethylamine 
• 28320-34-5 2-amino-7-bromo-9,9-dimethyl-9H-fluorene 
• 75-03-6 ethyl iodide 

Relevant academic research and scientific papers

Photostable and Orthogonal Solvatochromic Fluorophores for Simultaneous in Situ Quantification of Multiple Cellular Signaling Molecules

Ratiometric fluorescence sensors are powerful tools for direct quantification of diverse biological analytes. To overcome a shortage of solvatochromic fluorophores crucial for in situ ratiometric imaging of biological targets, we prepared and characterized a small library of modular fluorophores with diverse spectral properties. Among them, WCB and WCR showed excellent spectral properties, including high photostability, brightness, and solvatochromism, and are ideally suited for dual ratiometric imaging due to their spectral orthogonality. By conjugating WCB and WCR with protein-based lipid sensors, we were able to achieve robust simultaneous in situ quantitative imaging of two metabolically linked signaling lipids, phosphatidylinositol-4,5-bisphosphate and phosphatidylinositol-3,4,5-trisphosphate in live cells. This study shows that any combination of signaling molecules can be simultaneously quantified in a spatiotemporally resolved manner by ratiometric imaging with finely tuned solvatochromic fluorophores.

Air-Stable Pd Catalytic Systems for Sequential One-Pot Synthesis of Challenging Unsymmetrical Aminoaromatics

The selective functionalization of dibromoaromatic scaffolds using air-stable palladium catalytic systems was carried out. This methodology involved rapid mono and diselective Buchwald-Hartwig aminations via microwave irradiation. The conditions were optimized to couple sequentially different moieties in one pot. Couplings with a wide scope of amines allowed accessing a new library of symmetrical and unsymmetrical derivatives (35 examples). Using this versatile method, a near-IR push-pull sensor was prepared installing the electron-donating and -withdrawing groups through a multicomponent reaction. These conditions revealed to be gram-scalable and adaptable to various groups; hence, promoting facile use in synthetic chemistry.

Design, synthesis and properties of near-infrared molecular switches containing a fluorene ring

Three molecular switches containing a fluorene ring were designed and synthesized. The introduction of the amino group substituted fluorene ring resulted in the target molecular switches having some optical properties in the near-infrared region. It was demonstrated that the N-substituents on the fluorene rings and the switch units both had great influence on the molecular switch optical properties including the absorption maximum, absorption intensity and fluorescence quantum yield. The open-ring forms IA and IIA showed obvious solvatochromic behaviour. The closed-ring forms IIB and IIIB showed obvious hydrochromic behaviour in MeCN/water binary solvent systems and acidichromic behaviour in MeCN solution with high reversibility. Especially, the distinct off-on fluorescence signal in the near-infrared region using the stimuli of acid means that the designed compounds have great potential application value in the field of biological sensing.

Polarity-dependent photophysical properties of hemicyanine dyes and their application in 2-photon microscopy biological imaging

Two fluorescent hemicyanine type compounds, 1 and 2 have been developed for membrane staining, and their photophysical properties were investigated in various solvents. The demonstrated 2-photon-microscopy-biological-imaging application indicates the usefulness of 1 as a fluorescent probe.