1213779-13-5

Upstream product

• 125258-60-8 1-((2R,4S,5R)-5-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-hydroxytetrahydrofuran-2-yl)-5-methyl-2-thioxo-2,3-dihydropyrimidin-4(1H)-one 
• 1213779-12-4 1-(2'-deoxy-5'-O-4,4'-dimethoxytrityl-β-D-erythro-ribofuranosyl)-2-methylthiothymine 

Downstream Products

• 1213779-14-6 1-(2'-deoxy-5'-O-4,4'-dimethoxytrityl-β-D-erythro-ribofuranosyl)-2-cyanoethylselanylthymine 

Relevant academic research and scientific papers

Selenium-atom-modified thymidine enhances the specificity and sensitivity of DNA polymerization and detection

Nucleobase mismatches can jeopardize DNA polymerization specificity, causing mutations and errors in DNA replication and detection. Herein we report the first synthesis of novel 2-Se-thymidine triphosphate (SeTTP), describe the single-selenium

Selenium or thiothymine nucleoside-5 '-triphosphoric acid and synthesis method thereof

The invention discloses selenium or thiothymine nucleoside-5 '-triphosphoric acid and a synthesis method of the selenium or thiothymine nucleoside-5'-triphosphoric acid. The synthesis method comprises the following partial synthesis steps: a deprotection reaction is carried out on a compound 3 or 1 and trichloroacetic acid to respectively obtain SeT (namely 4Se) or ST (namely 4S); then, the compound 4Se and the compound 4S are respectively converted into a compound 5Se and a compound 5S through a one-pot synthesis method, SeTTP is the compound 5Se, and STTP is the compound 5S; next, the compounds 5Se and 5S are purified, the purified compounds 5Se and 5S are characterized to confirm the structure and purity of the compounds 5Se and 5S, and then DNA enzymatic synthesis is carried out by using SeTTP or STTP and DNA polymerase to obtain a compound 6, namely Se-DNA or S-DNA or selenium sulfo Se/S-DNA; according to the invention, a selenium or sulfur atom specific modification strategy SAM is established by innovatively synthesizing SeTTP and STTP and innovatively synthesizing DNA polymerase, so that T/G mismatch in DNA polymerization is inhibited, the specificity of base pairing is improved, and the SAM method is proved to be capable of improving the accuracy and sensitivity of polymerase reaction, nucleic acid molecule recognition and molecule detection.

MODIFIED NUCLEOTIDES AND METHODS FOR DNA AND RNA POLYMERIZATION AND SEQUENCING

Modified nucleotides, such as α-phosphoseleno-nucleotides (dNTPαSe and NTPαSe), can be incorporated into nucleic acids by enzymatic processes in a similar manner as naturally occurring nucleotides. Altering the properties of modified nucleotides can alter the interaction between the nucleotide and the enzyme. Enzymatic incorporation of modified nucleotides may occur at a lower rate than for native nucleotides, and can significantly inhibit misincorporation of nucleotides into nucleic acids during enzymatic extension and/or polymerization processes.

NOVEL COMPOUNDS AND DERIVATIZATION OF DNAS AND RNAS ON THE NUCLEOBASES OF PYRIMIDINES FOR FUNCTION, STRUCTURE, AND THERAPEUTICS

Disclosed are compounds of formula (I)3 a derivative, or a tautomer thereof, or a pharmaceutically acceptable salt of said compound or said tautomer. Also disclosed are methods of preparing compound of formula (I), a derivative, or a tautomer thereof, or