125224-45-5Relevant academic research and scientific papers
Highly selective purification of three lipases from Geotrichum candidum 4013 and their characterization and biotechnological applications
Brabcova, Jana,Demianova, Zuzana,Vondrasek, Jiri,Jagr, Michal,Zarevucka, Marie,Palomo, Jose M.
, p. 62 - 72 (2013/11/06)
Simple purification steps were used to purify two lipases (Lip1 and Lip2) and one novel lipase (Lip3) produced by Geotrichum candidum 4013. The octyl-Sepharose at low ionic strength was used to adsorbe Lip2 and novel Lip3, by addition of octadecyl-Sepabea
Lipase activity enhancement by SC-CO2 treatment
Hlavsova, Klara,Wimmer, Zdenek,Xanthakis, Epameinondas,Bernasek, Prokop,Sovova, Helena,Zarevucka, Marie
experimental part, p. 779 - 784 (2009/02/06)
The activity of lipases from porcine pancreas, Candida antartica recombinant from Aspergillus oryzae, Candida cylindracea (immobilized), Penicilium roqueforti, Aspergillus niger, Rhizopus arrhizus, Mucor miehei (two types of immobilization), and Pseudomonas cepacia (two types of immobilization) was studied after using them as biocatalysts of blackcurrant oil hydrolysis under SC-CO2 conditions. The reaction was performed at 40°C and 15 MPa in a continuous-flow reactor. Increased relative activity of all used lipases after the hydrolytic reaction was observed. The most remarkable increase in the activity was noted for the lipase from Rhizopus arrhizus which was increased by more than 50 times. The highest activity was shown by Lipozyme, lipase from Mucor miehei, immobilized on macroporous resin. Both treated and untreated Lipozyme were used as biocatalysts in hydrolytic resolution of the racemic cis- or frani-isomers of 2-(4-methoxybenzyl) cyclIohexyl acetates. Satisfactory reaction yields (40 %) and excellent enantiomeric purity of the products (E = 472) were obtained when hydrolysis of the frans-isomer of 2-(4-methoxybenzyl)cyclohexyl acetate was catalyzed by Lipozyme treated with SC-CO2.
Insect pest control agents: Novel chiral butanoate esters (juvenogens)
Wimmer, Zdenek,Floro, Alexandra J.F.D.M.,Zarevucka, Marie,Wimmerova, Martina,Sello, Guido,Orsini, Fulvia
, p. 6037 - 6042 (2008/03/28)
During the investigation of ester derivatives (juvenogens, biochemically activated insect hormonogenic compounds) of biologically active alcohols with potential application in insect pest control, a need for availability of all existing stereoisomers of e
Synthesis and structure assignment of 2-(4-methoxybenzyl)cyclohexyl β-D-glucopyranoside enantiomers
Saman, David,Kratina, Pavel,Moravcova, Jitka,Wimmerova, Martina,Wimmer, Zdenek
, p. 1470 - 1483 (2008/09/16)
Glucosylation of the cis- and trans-isomers of 2-(4-methoxybenzyl) cyclohexan-1-ol (1a/1b, 2a/2b, 1a or 2a) was performed to prepare the corresponding alkyl β-D-glucopyranosides, mainly to get analytical data of pure enantiomers of the glucosides (3a-6b),
Synthesis and structure assignment of 2-(4-methoxybenzyl)cyclohexyl β-D-galactopyranoside stereoisomers
Saman, David,Wimmerova, Martina,Wimmer, Zdenek
, p. 1186 - 1198 (2008/03/27)
Several promoters were used in the Koenigs-Knorr synthesis of the title alkyl β-D-galactopyranosides, both in their diastereoisomeric forms (5a/5b and 6a/6b), resulting from the synthesis performed with the respective racemic cis and trans isomers of 2-(4
Application of ionic liquids in enzymic resolution by hydrolysis of cycloalkyl acetates
Xanthakis, Epameinondas,Zarevucka, Marie,Saman, David,Wimmerova, Martina,Kolisis, Fragiskos N.,Wimmer, Zdenek
, p. 2987 - 2992 (2007/10/03)
A comparative study was performed in the enzymic resolution of the isomers of 2-(4-methoxybenzyl)cyclohexyl acetates 1 and 2. The investigation consisted in application of three commercially available lipases (Novozyme 435, Lipozyme IM and non-immobilized
Enzymic resolution of 2-substituted cyclohexanols through lipase-mediated esterification
Wimmer, Zdenek,Skouridou, Vasso,Zarevucka, Marie,Saman, David,Kolisis, Fragiskos N.
, p. 3911 - 3917 (2007/10/03)
Several lipases were used for the kinetic resolution of the racemic cis- and trans-isomers of 2-(4-methoxybenzyl)cyclohexanol, by lipase-mediated esterification of the substrates to the corresponding acetate isomers. Lipase from Rhizomucor miehei was foun
Synthesis and structure-activity relationships of juvenoids derived from 2-(4-hydroxybenzyl)cycloalkan-1-ones
Rejzek,Wimmer,Saman,Ricankova,Nemec
, p. 1241 - 1255 (2007/10/02)
Juvenoids 16-30, 32, 36, 38-41, 44-46, and 49-56 containing carbamate, carbonate, and urea moieties in the molecule were synthesized and subjected to a biological screening. Carbamate juvenoids 1-4 were used as reference compounds for a detailed structure-activity study of their analogues. A clear relationship between the nature of the side chain functional group and the biological activity was found. Surprisingly, not only the juvenoids 1,4 but also 38-41, the compounds with a reversed carbamate N,O-substitution pattern, showed very promising biological activity. In contrast, the carbonate and urea derivatives displayed a remarkably low activity. The relationship between the size and substitution at atoms C(2) and C(3) of the saturated ring and the biological activity is very complex and is still not completely understood.
BIOTRANFORMATION OF 2-(4-METHOXYBENZYL)-1-CYCLOHEXANONE BY MEANS OF Saccharomyces cerevisiae
Wimmer, Zdenek,Budesinsky, Milos,Macek, Tomas,Svatos, Ales,Saman, David,et.al.
, p. 2326 - 2337 (2007/10/02)
Biotranformation reduction of 2-(4-methoxybenzyl)-1-cyclohexanone was investigated.A method was elaborated for the preparation of some diastereoisomeric alcohols derived from the racemic title ketone and for the determination of the products obtained.The
CONFORMATION STUDIES OF ISOMERIC 1-ACYLOXY-2-(4-METHOXYBENZYL)-CYCLOHEXANES BY 1H NMR SPECTTOSCOPY
Budesinsky, Milos,Saman, David,Wimmer, Zdenek
, p. 2763 - 2767 (2007/10/02)
The conformation of the cyclohexane ring and the p-methoxybenzyl substituent in the series of isomeric 1-acyloxy-2-(4-methoxybenzyl)cyclohexanes was studied by means of 1H NMR spectroscopy.In all the substances investigated the cyclohexane ring assumes ch
