125258-63-1

Upstream product

• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 28585-51-5 2-thiothymidine 
• 124482-92-4 2-cyanoethyl-N,N-(diisopropylamino)chlorophosphine 
• 125258-60-8 1-((2R,4S,5R)-5-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-hydroxytetrahydrofuran-2-yl)-5-methyl-2-thioxo-2,3-dihydropyrimidin-4(1H)-one 

Downstream Products

• 1350852-16-2 C₄₂H₅₀N₇O₆PS 
• 1350852-17-3 C₄₀H₅₀N₅O₆PS 
• 1350852-11-7 C₄₇H₅₄N₅O₇PS 

Relevant academic research and scientific papers

Fluorescent labeling of s2T-incorporated DNA and m5s2U-modified RNA

We report herein comprehensive investigations of alkylation/sulfur exchange reactions of sulfur-containing substrates including nucleosides such as s2U, m5s2U, s4U, s2A and s2T-incorporated DNA enable by comprehensive screenings of the reagents (2a–2h). It has been proven that iodoacetamide (2a) displays the most promising feasibility toward sulfur-containing substrates including s2T, s2U, m5s2U, s4U and s2A. In sharp contrast, the alkylation process with S-benzyl methanethiosulfonate (BMTS, 2h) displays the best application potential only for s4U. Based on these results, the fluorescent labeling of s2T-incorporated DNA and m5s2U-modified RNA has been achieved. Supplemental data for this article is available online at https://doi.org/10.1080/15257770.2021.1942044.

The synthesis of oligodeoxynucleotides containing 2-thiothymine and 5-methyl-4-pyrimidinone base analogues

A procedure is described for the preparation of two thymidine analogues in which the O2-carbonyl is deleted (dH2T) or converted to an O2-thione (ds2T). The phosphoramidites of these nucleosides have been synthes