1284183-64-7Relevant academic research and scientific papers
Pyraclostrobin hapten, artificial antigen and antibody as well as preparation method and application thereof
-
Paragraph 0036; 0038-0040, (2020/12/06)
The invention discloses a pyraclostrobin hapten, an artificial antigen, an antibody as well as a preparation method and application thereof. The provided pyraclostrobin hapten retains the characteristic structure of pyraclostrobin to the greatest extent, so that the immunogenicity of the pyraclostrobin hapten is obviously enhanced and the pyraclostrobin hapten has a carboxyl coupled with a carrierprotein; a pyraclostrobin artificial antigen obtained by coupling the pyraclostrobin hapten with a carrier protein is used for immunizing animals; the prepared pyraclostrobin antigen is more beneficial to stimulating animal immune response to generate an antibody with stronger specificity and higher sensitivity, the sensitivity of the pyraclostrobin antibody can reach 0.1 [mu]g/L through detection, the cross reaction rate of the pyraclostrobin antibody and other methoxy acrylate bactericides is low, and a basis is provided for establishing various immunoassay methods of pyraclostrobin subsequently.
Synthesis of site-heterologous haptens for high-affinity anti-pyraclostrobin antibody generation
Mercader, Josep V.,Agullo, Consuelo,Abad-Somovilla, Antonio,Abad-Fuentes, Antonio
, p. 1443 - 1453 (2011/04/22)
The design and synthesis of functional chemical derivatives of small organic molecules is usually a key step for the intricate production of a variety of bioconjugates. In this respect, the derivatization site at which the spacer arm is introduced in immunizing conjugates constitutes a highly critical parameter for the generation of high-affinity and selective antibodies. However, due to the usual complexity of the required synthetic procedures, the appropriate comparison of alternative tethering positions has often been neglected. In the present study, meticulous strategies were followed to prepare synthetic derivatives of pyraclostrobin with the same linkers located at diverse rationally-chosen sites. Activity appraisal of antibodies and bioconjugates was carried out by bidimensional competitive direct and indirect immunoassays, and a superior performance of two of the three synthesized haptens was found. Finally, a detailed analysis of the conformations of the target molecule and the synthesized haptens in aqueous solution was done using computer assisted molecular modeling techniques. This study suggested that the lower titers and affinities of one set of antibodies are most probably due to conformational effects of the spacer arm in the immunizing bioconjugate. The Royal Society of Chemistry 2011.
