129451-77-0 Usage
Uses
Used in Synthetic RNA Preparation:
5'-O-(4,4'-DiMethoxy)3'-O-(t-butyldiMethylsilyl) uridine, 2'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoraMidite is used as a building block for the preparation of synthetic RNA in the solid-phase synthesis process. The compound's unique structure allows for the efficient and controlled synthesis of RNA molecules with specific sequences, which can be used for various applications in research and therapeutics.
Used in Chemical Synthesis and Modification of RNA Molecules:
In the field of chemical synthesis, this uridine derivative can be used as a key intermediate for the modification of RNA molecules. The presence of the phosphoramidite group enables the compound to be incorporated into longer RNA sequences through automated solid-phase synthesis. The other functional groups, such as the 5'-O-(4,4'-dimethoxy) and 3'-O-(t-butyldiMethylsilyl) groups, provide additional sites for further chemical modifications, allowing for the creation of RNA molecules with tailored properties and functions.
Used in Pharmaceutical Research:
The unique structure of 5'-O-(4,4'-DiMethoxy)3'-O-(t-butyldiMethylsilyl) uridine, 2'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoraMidite may also make it a valuable compound in pharmaceutical research. Its ability to be incorporated into synthetic RNA molecules could potentially be exploited for the development of new drugs targeting RNA-based therapeutics, such as antisense oligonucleotides or RNA interference (RNAi) therapies.
Used in Biomedical Research:
In biomedical research, this uridine derivative can be utilized for the study of RNA structure, function, and interactions with other biomolecules. The modified uridine can be incorporated into RNA molecules to investigate the effects of specific modifications on RNA stability, folding, and binding to proteins or other RNA molecules. This information can be valuable for understanding the role of RNA in various biological processes and for the development of new diagnostic and therapeutic tools.
Used in Nanotechnology and Materials Science:
The unique chemical structure of 5'-O-(4,4'-DiMethoxy)3'-O-(t-butyldiMethylsilyl) uridine, 2'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoraMidite may also find applications in the fields of nanotechnology and materials science. The compound's functional groups could potentially be used to create self-assembling structures or to functionalize surfaces with specific properties, such as enhanced biocompatibility or the ability to bind specific target molecules.
Check Digit Verification of cas no
The CAS Registry Mumber 129451-77-0 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,2,9,4,5 and 1 respectively; the second part has 2 digits, 7 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 129451-77:
(8*1)+(7*2)+(6*9)+(5*4)+(4*5)+(3*1)+(2*7)+(1*7)=140
140 % 10 = 0
So 129451-77-0 is a valid CAS Registry Number.
129451-77-0Relevant academic research and scientific papers
Solid-phase synthesis and hybrization behavior of partially 2′/3′-O-acetylated RNA oligonucleotides
Xu, Jianfeng,Duffy, Colm D.,Chan, Christopher K. W.,Sutherland, John D.
, p. 3311 - 3326 (2014/05/06)
Synthesis of partially 2′/3′-O-acetylated oligoribonucleotides has been accomplished by using a 2′/3′-O-acetyl orthogonal protecting group strategy in which non-nucleophilic strong-base (DBU) labile nucleobase protecting groups and a UV-light cleavable linker were used. Strong-base stability of the photolabile linker allowed on-column nucleobase and phosphate deprotection, followed by a mild cleavage of the acetylated oligonucleotides from the solid support with UV light. Two 17nt oligonucleotides, which were synthesized possessing one specific internal 2′- or 3′-acetyl group, were used as synthetic standards in a recent report from this laboratory detailing the prebiotically plausible ligation of RNA oligonucleotides. In order to further investigate the effect of 2′/3′-O-acetyl groups on the stability of RNA duplex structure, two complementary bis-acetylated RNA oligonucleotides were also expediently obtained with the newly developed protocols. UV melting curves of 2′-O-acetylated RNA duplexes showed a consistent ～3.1 °C decrease in Tm per 2′-O-acetyl group.
On Facts and Artefacts: The Difficulty to Evaluate an Artificial Nuclease
Pitsch, Stefan,Scheffer, Ute,Hey, Markus,Strick, Andreas,Goebel, Michael W.
, p. 3740 - 3752 (2007/10/03)
A number of promising synthetic catalysts for the hydrolytic degradation of RNA have been developed in recent years. Some of them show remarkable selectivity for pyrimidine nucleotides. The general problem of all these studies is to distinguish between real effects and artefacts caused by traces of contaminating natural ribonucleases. We show that methods representing the current state of the art (diethylpyrocarbonate treatment, sterilization, ultrafiltration, etc.) do not sufficiently protect against severe artefacts. However, an incorruptible assay could be found by comparing the cleavage of RNA and its mirror image. Enantiomeric RNA is completely resistant to enzymatic degradation, whereas achiral nonpeptide catalysts, by fundamental laws of symmetry, cannot distinguish between enantiomers and will induce exactly the same cleavage pattern with both substrates.
