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133-32-4 Usage


3-Indolebutyric acid, also known as Indole-3-butyric acid (IBA), is a plant hormone belonging to the auxin family. It is a white to light yellow crystalline solid with a slight characteristic odor. IBA is widely used in agriculture due to its ability to induce rooting in various plant species and acts as a precursor to the most abundant and potent native auxin, indole-3-acetic acid (IAA), which generates the majority of auxin effects in intact plants.


Used in Agriculture:
3-Indolebutyric acid is used as a plant growth regulator for promoting the cuttings and rooting of herbaceous and woody ornamental plants. It enhances the fruit setting of fruits and improves the fruit setting rate, making it a valuable tool in horticulture and agriculture.
Used in Plant Cell Culture:
IBA is used as a rooting agent to stimulate root formation of plant clippings, making it suitable for plant cell culture testing and applications.
Used in Plant Hormone Research:
As an auxin-family plant hormone, IBA is used in research to study the role of auxins in plant growth and development, as well as their potential applications in various agricultural and horticultural practices.
Chemical Properties:
The pure product of 3-Indolebutyric acid is white crystals with a melting point of 124-125°C and a vapor pressure of less than 10 × 10^-6 Pa at 60°C. It is easily soluble in organic solvents but insoluble in water. IBA is stable to acid, and the industrial product is white to pale yellow crystals with a melting point of 121-124°C. The solubility of IBA in water is 250 mg L^-1.

Plant Growth Regulator

Indole-3-butyric acid (IBA) is a plant hormone belonging to the auxin family and assists in initiating root formation; the in vitro process is called micropropagation. Aside from accelerating root formation, it is used on various crops to stimulate flower development and the growth of fruits. This ultimately increases crop yields.Because it is similar in structure to naturally occurring substances and is used in tiny amounts, this plant growth regulator poses no known risks to humans or the environment.Indole-3-butyric acid is not easy to be oxidized in plants and has a poor conductivity. It has similar physiological effect as indole acetic acid. It can take effect in cell division and cell growth of plants but with a less significant effect compared with indole acetic acid is mainly used to promote rooting cuttings and effectively promote cell division cambium. It has a relative long maintaining time for its efficacy and can promote more but slender adventitious roots with better efficacy when being administered together with naphthalene acetic acid.Indole-3-butyric acid can be applied to the cuttings and root cutting of chrysanthemum and other ornamental plants for facilitating the rooting at a concentration of 0.5~1.0mg/L. However, we should not be applied to the foliage of the plants. Degradation and Metabolism: it can subject to rapid degradation in the soil.

Physical and Chemical Properties

3-Indolebutyric acid: (molecule formula: C11H12O2N) has a molecular weight of 190.22. The pure product is white crystalline solid with the melting point being 124~125 ℃. Its industrial product is white to pale yellow crystals with a melting point being 121~124 ℃. It has special smell and is toxic! It is irritant with a vapor pressure being <10μPa at 60 ℃. It is difficult to be dissolved in water with the solubility in water at 20 ℃ being 0.25g/L. It is easily soluble in benzene and soluble in other organic solvents. Its solubility (g/100ml) is: benzene> 100; acetone, ethanol, ethyl ether: 3 to 10; chloroform: 1 to 10. 3-indolebutyric acid has a low toxicity to humans and animals and is stable to the acid while forming salt in the solution of the hydroxide and carbonate compounds of the alkali metal. It can be produced from indole via Grignard reaction or by adding nitrile for hydrolysis. In Agriculture, 3-indolebutyric acid can be used as plant growth-promoting agent that can promote root growth and fruit ripening with an efficacy being stronger than indole acetic acid. Indole butyric acid is an excellent growth regulator for promoting the cutting and rooting of the cherry rootstock. You can apply rapid high-concentration dip method or soaking for treatment of cutting slips. Rapid high-concentrations dip method is through dipping the base of the cutting slips in IBA solution of a concentration of 5 × 10-4~1 × 10-2 for 5 seconds and immediately inserting the cutting slip in the bed. This method has the advantage of short processing time, even medication and excellent efficacy. After the treatment, adding simazine to the insertion beds (20.375 grams per meter) can lead to a higher rate of rooting.


Laboratory put the mixture of indole, potassium hydroxide, and poly oxalic acid, dried tetrahydronaphthalene and γ-butyrolactone for heating under reflux to obtain the indole butyric acid. The reaction equation is as follows: Indole, potassium hydroxide, poly oxalic acid and dried tetrahydronaphthalene were added to the reaction vessel. The mixture was stirred for half an hour at room temperature; add γ-butyrolactone for heating reflux for 4 hours. The water remaining in the reaction system is removed by the water segregator. After cooling, the solid was totally dissolved by water with the separated organic layer being acidified with hydrochloric acid for precipitating a large number of off-white solid. Use suction filtration, wash with water, and dry to give the crude indole butyric acid with the yield of the crude product being 92.6%. After recrystallisation but aqueous ethanol, you can obtain white flaky crystals that is indole-3-butyric acid with the mp being 121.5~123 ℃. Reference: Daquan Wang (editor), “fine chemical production route” 2nd version, Beijing: Chemical Industry Press .1999 p. 260-261.

Analytical method

1. Product analysis: in the absolute ethanol, measure it at wavelength of 281nm through UV spectrophotometry (ACF Chemiefarma method). 2. Residue Analysis: use chloroform for extraction from the acid medium, and put its methanol solution together with petroleum ether (40~60 ℃) for shaking absorption, and further use high performance liquid chromatography for applying ultraviolet detection (ACF Chemiefarma France). Alternatively, you can also refer to the indole acetic acid residue analysis. First derive methyl or the trimethyl ester of silane, and then use gas chromatography for analysis.


It has low toxicity to humans and animals. Acute-oral rat: LD50: 5000mg/kg, mice 1760mg/kg. Carp LC50: 180mg/L (48h). It has low toxicity to bees.

Biochem/physiol Actions

Indole-3-butyric acid (IBA) is a naturally occurring phytohormone auxin (plant growth regulator). It promotes root formation in cuttings but does not affect ethylene levels. IBA might be a precursor for indole-3-acetic acid (IAA) through β oxidation pathway. IBA is present in plant species like Zea mays, Pisum sativum and Arabidopsis. IBA is more potent than IAA for inducing root formation.

Purification Methods

Recrystallise the acid from H2O. It is soluble in EtOH, Et2O and Me2CO but insoluble in CHCl3. [Bowman & Islip Chem Ind London 154 1971, Jackson & Manske J Am Chem Soc 52 5029 1930, Albaum & Kaiser Am J Bot 24 420 1937.] It has also been recrystallised from EtOH/water [James & Ware J Phys Chem 89 5450 1985]. Its UV has 278 and 320nm in isoPrOH [Elvidge Quart J Pharm Pharmacol 13 219 1940].max The methyl ester has m 73-74o (from *C6H6/pet ether) and b 230o/6mm [Bullock & Hand J Am Chem Soc 78 5854 1951]. [Beilstein 22 III/IV 1128, 22/3 V 140.]

Check Digit Verification of cas no

The CAS Registry Mumber 133-32-4 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 1,3 and 3 respectively; the second part has 2 digits, 3 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 133-32:
34 % 10 = 4
So 133-32-4 is a valid CAS Registry Number.

133-32-4 Well-known Company Product Price

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  • (Code)Product description
  • CAS number
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  • Detail
  • TCI America

  • (I0026)  3-Indolebutyric Acid  >98.0%(HPLC)(T)

  • 133-32-4

  • 5g

  • 96.00CNY

  • Detail
  • TCI America

  • (I0026)  3-Indolebutyric Acid  >98.0%(HPLC)(T)

  • 133-32-4

  • 25g

  • 215.00CNY

  • Detail
  • Alfa Aesar

  • (A15260)  Indole-3-butyric acid, 98%   

  • 133-32-4

  • 5g

  • 331.0CNY

  • Detail
  • Alfa Aesar

  • (A15260)  Indole-3-butyric acid, 98%   

  • 133-32-4

  • 25g

  • 992.0CNY

  • Detail
  • Alfa Aesar

  • (A15260)  Indole-3-butyric acid, 98%   

  • 133-32-4

  • 100g

  • 3472.0CNY

  • Detail
  • Sigma-Aldrich

  • (45532)  Indole-3-butyricacid  PESTANAL®, analytical standard

  • 133-32-4

  • 45532-250MG

  • 450.45CNY

  • Detail



According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 10, 2017

Revision Date: Aug 10, 2017


1.1 GHS Product identifier

Product name indole-3-butyric acid

1.2 Other means of identification

Product number -
Other names beta-indolebutyricacid

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:133-32-4 SDS

133-32-4Related news

Root hair development of Poncirus trifoliata grown in different growth cultures and treated with 3-Indolebutyric acid (cas 133-32-4) and ethephon10/01/2019

Citrus plants are generally believed to possess few or even no root hair whose function is to uptake nutrient and water from soils. This study compared the development of root hair of a citrus rootstock, Poncirus trifoliata, in four types of cultures or treated with 3-indolebutyric acid and ethe...detailed

133-32-4Relevant articles and documents


Avramenko et al.

, (1970)



Avramenko,V.G. et al.

, (1973)


Pd-Catalyzed Highly Chemo- And Regioselective Hydrocarboxylation of Terminal Alkyl Olefins with Formic Acid

Ren, Wenlong,Chu, Jianxiao,Sun, Fei,Shi, Yian

supporting information, p. 5967 - 5970 (2019/08/26)

An efficient Pd-catalyzed hydrocarboxylation of alkenes with HCOOH is described. A wide variety of linear carboxylic acids bearing various functional groups can be obtained with excellent chemo- and regioselectivities under mild reaction conditions. The reaction process is operationally simple and requires no handling of toxic CO.

Deciphering DNA-based asymmetric catalysis through intramolecular Friedel-Crafts alkylations

Park, Soyoung,Ikehata, Keiichi,Watabe, Ryo,Hidaka, Yuta,Rajendran, Arivazhagan,Sugiyama, Hiroshi

, p. 10398 - 10400,3 (2020/09/09)

We describe asymmetric intramolecular Friedel-Crafts alkylations with a DNA-based hybrid catalyst and propose a plausible binding model. This study shows promise for studying relationships between the helical chirality of DNA and enantioselectivity of the chemical reaction.

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