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Usage

Uses

Used in Bioconjugation Applications:
Diazo Biotin-PEG3-Azide is used as a linker for bioconjugation, enabling the attachment of biotin to alkyne-containing biomolecules. This facilitates the subsequent capture of these biomolecules by streptavidin, which is useful in various research and diagnostic applications.
Used in Streptavidin-Biotin Interactions:
In the field of molecular biology, Diazo Biotin-PEG3-Azide is used as a tool to study streptavidin-biotin interactions. The biotin moiety can be captured by streptavidin, allowing for the investigation of binding kinetics and the development of assays.
Used in Bioanalytical Techniques:
Diazo Biotin-PEG3-Azide is employed as a component in the development of bioanalytical techniques, such as enzyme-linked immunosorbent assays (ELISA) and biosensors, where the biotin-streptavidin interaction is utilized for signal amplification and detection.
Used in Drug Delivery Systems:
In pharmaceutical research, Diazo Biotin-PEG3-Azide can be used as a component in drug delivery systems, where the biotin-streptavidin interaction can be leveraged for targeted drug delivery and controlled release.
Used in Materials Science:
In materials science, Diazo Biotin-PEG3-Azide can be utilized for the development of biocompatible materials and surfaces, where the biotin-streptavidin interaction can be employed for immobilizing biomolecules or creating self-assembling structures.

Upstream product

• 359860-27-8 N-(2-(2-(2-(2-amidoethoxy)ethoxy)ethoxy)ethoxy)biotinamide 
• 875770-34-6 N-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethyl]biotinamide 

Relevant academic research and scientific papers

Chemical modification and organelle-specific localization of Orlistat-like natural-product-based probes

Orlistat, also known as tetrahydrolipstatin (THL), is an FDA-approved anti-obesity drug with potential anti-cancer activity. Previously, we developed a chemical proteomic approach, based on the Orlistat-like probe (1a) for large-scale identification of unknown cellular targets of Orlistat in human hepatocytes. In this article, we report the chemical synthesis and biological evaluation of an expanded set of Orlistat-like compounds, with the intention to further dissect and manipulate potential cellular targets of Orlistat. In doing so, we carried out proteome-wide activity-based profiling and large-scale pull-down/LCMS analysis of these compounds in live HepG2 cells, and successfully identified many putative cellular targets for Orlistat and its structural analogues. By qualitatively assessing the spectra counts of potential protein hits against each of the seventeen Orlistat analogues, we obtained both common and unique targets of these probes. Our results revealed that subtle structural modifications of Orlistat led to noticeable changes in both the cellular potency and target profiles of the drug. In order to further improve the cellular activity of Orlistat, we successfully applied the well-established AGT/SNAP-tag technology to our cell-permeable, benzylguanine (BG)-containing Orlistat variant (4). We showed that the drug could be delivered and effectively retained in different sub-cellular organelles of living cells. This strategy may provide a general and highly effective chemical tool for the potential sub-cellular targeting of small molecule drugs.