136205-88-4Relevant academic research and scientific papers
Fluorescent and colorimetric detection of acid vapors by using solid-supported rhodamine hydrazides
Kang, Shincheol,Kim, Sungwook,Yang, Young-Keun,Bae, Shinhyo,Tae, Jinsung
, p. 2010 - 2012 (2009)
Rhodamine hydrazide-based chemosensors that can detect volatile acidic gases in solid state have been developed. The rhodamine hydrazide probes adsorbed on filter paper respond fluorescently and colorimetrically only to volatile acidic gases but not to organic bases nor to other volatile organic compounds (VOCs). Diethyl chlorophosphate (DCP), one of model compounds used for the studies of Chemical Warfare Agent (CWA), could also be detected by using this method.
Endoplasmic Reticulum Targeting Reactive Oxygen Species Sensor Based on Dihydrofluorescein: Application of Endoplasmic Reticulum Stress
Le, Hoa Thi,Jo, Hye-Ryeong,Oh, Se-Yun,Jung, Jinwook,Kim, Young Gi,Kang, Chulhun,Kim, Tae Woo
, p. 279 - 285 (2021)
Endoplasmic reticulum (ER) has a unique redox environment, which plays critical roles in the organelle's function and in its pathological responses such as ER stress. In this work, we introduce an ER-targeting fluorogenic reactive oxygen species (ROS) chemosensor (ER-Flu) from copper(I)-catalyzed alkyne-azide cycloaddition of 3-propargyl ester of 2′,7′-dichlorodihydrofluorescein diacetate and N3-glibenclamide, which were adopted as a fluorogenic ROS sensing module and an ER-targeting module, respectively. Thereby, a series of confocal microscopic experiments of ER-Flu demonstrated that the sensor localizes in ER of the live cells and that ROS are elevated in the cells by ER stress inducers such as thapsigargin, brefeldin A, and tunicamycin.
Preparation method of kit and application of kit to protein fluorescence labeling
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Paragraph 0041-0046, (2020/12/09)
The invention discloses a preparation method of a kit and application of the kit in protein fluorescence labeling, and particularly relates to the technical field of protein detection. The preparationmethod comprises the following steps: (1) synthesis of fluorescein methyl ester; (2) synthesis of 6-oxo-monomaleate monoester-9-(2'-methoxycarbonyl)fluorescein; and (3) synthesis of the 6-oxo-(N-hydroxysuccinimide maleic acid monoester)-9-(2'-methoxycarbonyl)fluorescein. The preparation method can be used for preparing a novel protein fluorescence labeling reagent, and the novel protein fluorescence labeling reagent has the advantages of high labeling product stability, high labeling efficiency, high fluorescence quantum yield, short labeling time and simple reaction conditions during labeling.
