1372118-47-2Relevant academic research and scientific papers
A two-photon fluorescent probe for viscosity imaging in vivo
Ning, Peng,Dong, Pengyu,Geng, Qian,Bai, Lei,Ding, Yaqi,Tian, Xiaohe,Shao, Rong,Li, Lin,Meng, Xiangming
, p. 2743 - 2749 (2017)
A new two-photon fluorescent probe (MCN) for viscosity imaging was developed based on a 6-substituted quinoline framework. MCN showed an excellent “off-on” fluorescence response (ca. 90-fold enhancement) with viscosity increasing in the glycerol-water viscosity system. MCN showed great sensitivity to viscosity (R2 = 0.98, x = 0.65), which gave rise to cell imaging for micro-viscosity or real-time cell imaging during apoptosis with low cytotoxicity under two-photon excitation (λex = 800 nm). Fluorescence lifetime imaging (FLIM) of living HeLa cells stained with MCN revealed that the intracellular average viscosity value was 73.45 ± 21.55 cP in cytosol. Imaging in living tissue slices indicated that MCN can work in deep tissue (～130 μM) under two-photon excitation. Moreover, MCN also showed the capacity for in vivo imaging viscosity in zebrafish with obvious fluorescence emission.
6-Substituted quinoline-based ratiometric two-photon fluorescent probes for biological Zn2+ detection
Meng, Xiangming,Wang, Shuxin,Li, Yiming,Zhu, Manzhou,Guo, Qingxiang
, p. 4196 - 4198 (2012/05/20)
New ratiometric two-photon fluorescent probes are developed from 6-substituted quinolines for biological Zn2+ detection. They show large red shifts and good ratiometric responses upon Zn2+ binding. They also exhibit high ion selectivities and large two-photon absorption cross sections at nearly 720 nm. Because the new probes are cell-permeable, they can be used to detect intracellular zinc flux under two-photon excitation.
