1436409-49-2

Upstream product

• 428865-77-4 4-methoxyphenyl 2-O-benzyol-3-O-benzyl-β-D-glucopyranoside 
• 1436409-46-9 benzyl (4-methoxyphenyl 2-O-benzoyl-3-O-benzyl-β-D-glucopyranoside)uronate 
• 123-76-2 levulinic acid 

Downstream Products

• 1436409-51-6 benzyl 2-O-benzoyl-3-O-benzyl-4-O-levulinoyl-α-D-glucopyranosuronate 
• 1436409-53-8 benzyl 2-O-benzoyl-3-O-benzyl-4-O-levulinoyl-β-D-glucopyranosuronate 

Relevant academic research and scientific papers

Synthesis of chondroitin/dermatan sulfate-like oligosaccharides and evaluation of their protein affinity by fluorescence polarization

Here, we present a novel approach for the chemical synthesis of chondroitin and dermatan sulfate oligosaccharides. A key point of this strategy is the preparation and use of an N-trifluoroacetyl galactosamine building block containing a 4,6-O-di-tert-butylsilylene group. Glycosylation reactions proceeded in good yields (74-91%) with our protecting group distribution. Using this approach, we have synthesized, for the first time, a chondroitin/dermatan sulfate-like tetrasaccharide that contains both types of uronic acids, d-glucuronic and l-iduronic acid. Moreover, we have employed a fluorescence polarization competition assay to evaluate the interactions between the synthesized oligosaccharides and FGF-2 (basic fibroblast growth factor). Our results show that this method, using standard instrumentation and minimal sample consumption, is a powerful tool for the rapid analysis of the glycosaminoglycan affinity for proteins in solution. The Royal Society of Chemistry.