1449-72-5Relevant articles and documents
The Chemical Shift Baseline for High-Pressure NMR Spectra of Proteins
Frach, Roland,Kibies, Patrick,B?ttcher, Saraphina,Pongratz, Tim,Strohfeldt, Steven,Kurrmann, Simon,Koehler, Joerg,Hofmann, Martin,Kremer, Werner,Kalbitzer, Hans Robert,Reiser, Oliver,Horinek, Dominik,Kast, Stefan M.
supporting information, p. 8757 - 8760 (2016/07/21)
High-pressure (HP) NMR spectroscopy is an important method for detecting rare functional states of proteins by analyzing the pressure response of chemical shifts. However, for the analysis of the shifts it is mandatory to understand the origin of the observed pressure dependence. Here we present experimental HP NMR data on the15N-enriched peptide bond model, N-methylacetamide (NMA), in water, combined with quantum-chemical computations of the magnetic parameters using a pressure-sensitive solvation model. Theoretical analysis of NMA and the experimentally used internal reference standard 4,4-dimethyl-4-silapentane-1-sulfonic (DSS) reveal that a substantial part of observed shifts can be attributed to purely solvent-induced electronic polarization of the backbone. DSS is only marginally responsive to pressure changes and is therefore a reliable sensor for variations in the local magnetic field caused by pressure-induced changes of the magnetic susceptibility of the solvent.
