1449482-16-9

Upstream product

• 1449482-14-7 5-(5’-O-dimethoxytrityl-2’-deoxy-D-ribofuranosyl)-2-phenoxyacetylaminopyridine 
• 124482-92-4 2-cyanoethyl-N,N-(diisopropylamino)chlorophosphine 
• 950830-66-7 2-amino-5-[3’,5’-di-O-tert-butyldimethylsilyl-(2’-deoxy-D-ribofuranosyl)]pyridine 
• 1449482-10-3 5-[3’,5’-di-O-tert-butyldimethylsilyl-(2’-deoxy-D-ribofuranosyl)]-2-phenoxyacetylaminopyridine 

Relevant academic research and scientific papers

A 2-amino-6-methylpyridin-5-yl nucleobase for GC base pair recognition in the parallel triplex DNA

DNA triple helices containing consecutive C+H·GC triplets are unstable at neutral pH because protonations of cytosines into the third strand are necessary. Previously, a 2-aminopyridin-5-yl nucleobase (P) was developed and has been widely used as a substitute for the cytosine nucleobase. In this work, we designed a 2-amino-6-methylpyridin-5-yl nucleobase (PMe), which could preferentially adopt an anti-orientation by the 6-methyl group. This might lead to formation of a stable base triplet with a GC base pair compared to P. We also synthesized 15-mer triplex-forming oligonucleotides (TFOs) containing PMe by the standard solid-phase method and evaluated the triplex stability of the TFOs at neutral pH by UV melting experiments.