149559-87-5Relevant academic research and scientific papers
Synthesis of cyclic di-nucleotidic acids as potential inhibitors targeting diguanylate cyclase
Ching, Shi Min,Tan, Wan Jun,Chua, Kim Lee,Lam, Yulin
experimental part, p. 6657 - 6665 (2010/10/21)
Five analogs of cyclic di-nucleotidic acid including c-di-GMP were synthesized and evaluated for their biological activities on Slr1143, a diguanylate cyclase of Synechocystis sp. Slr1143 was overexpressed from the recombinant plasmid which contained the gene of interest and subsequently purified by affinity chromatography. A new HPLC method capable of separating the compound and product peaks with good resolution was optimized and applied to the analysis of the compounds. Results obtained show that cyclic di-inosinylic acid 1b demonstrates a stronger inhibition on Slr1143 than c-di-GMP and is a potential inhibitor for biofilm formation.
An improved method for synthesizing cyclic bis(3′-5′)diguanylic acid (c-di-GMP)
Hyodo, Mamoru,Hayakawa, Yoshihiro
, p. 2089 - 2093 (2007/10/03)
This paper describes a new method for synthesizing biologically important cyclic bis(3′-5′)diguanylic acid (c-di-GMP) in a higher yield than that previously reported to be available by our synthetic method. In the new synthesis, the following two means, i
