150374-99-5Relevant academic research and scientific papers
Leveraging a "catch-Release" Logic Gate Process for the Synthesis and Nonchromatographic Purification of Thioether- or Amine-Bridged Macrocyclic Peptides
Kheirabadi, Mahboubeh,Creech, Gardner S.,Qiao, Jennifer X.,Nirschl, David S.,Leahy, David K.,Boy, Kenneth M.,Carter, Percy H.,Eastgate, Martin D.
, p. 4323 - 4335 (2018/04/25)
Macrocyclic peptides containing N-alkylated amino acids have emerged as a promising therapeutic modality, capable of modulating protein-protein interactions and an intracellular delivery of hydrophilic payloads. While multichannel automated solid-phase peptide synthesis (SPPS) is a practical approach for peptide synthesis, the requirement for slow and inefficient chromatographic purification of the product peptides is a significant limitation to exploring these novel compounds. Herein, we invent a "catch-release" strategy for the nonchromatographic purification of macrocyclic peptides. A traceless catch process is enabled by the invention of a dual-functionalized N-terminal acetate analogue, which serves as a handle for capture onto a purification resin and as a leaving group for macrocyclization. Displacement by a C-terminal nucleophilic side chain thus releases the desired macrocycle from the purification resin. By design, this catch/release process is a logic test for the presence of the key components required for cyclization, thus removing impurities which lack the required functionality, such as common classes of peptide impurities, including hydrolysis fragments and truncated sequences. The method was shown to be highly effective with three libraries of macrocyclic peptides, containing macrocycles of 5-20 amino acids, with either thioether- or amine-based macrocyclic linkages; in this latter class, the reported method represents an enabling technology. In all cases, the catch-release protocol afforded significant enrichment of the target peptides purity, in many cases completely obviating the need for chromatography. Importantly, we have adapted this process for automation on a standard multichannel peptide synthesizer, achieving an efficient and completely integrated synthesis and purification platform for the preparation of these important molecules.
Method of synthesizing [...]
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Paragraph 0003; 0006; 0009; 0032; 0033, (2016/12/16)
PURPOSE: A method for synthesizing sivelestat is provided to perform reaction without separation of intermediates and to improve yield and productivity. CONSTITUTION: A method for synthesizing sivelestat comprises the steps of: (i) reacting 4-pivaloyloxy benzenesulfonate salts with halogenating agents to obtain 4-(halosulfonyl)phenyl pivalate; (ii) reacting isatoic anhydride with benzyl glycinate to obtain benzyl 2-(2-aminobenzamido)acetate; (iii) reacting the products obtained from steps (i) and (ii) to obtain 4-(N-(2-(2-(benzyloxy)-2-oxoethylcarbamoyl)phenyl)sulfamoyl)phenyl pivalate; and (iv) performing debenzylation of the product of step (iii) to obtain sivelestat.
Synthesis and pharmacological characterization of 2-aminobenzaldehyde oxime analogs as dual inhibitors of neutrophil elastase and proteinase 3
Hwang, Tsong-Long,Wang, Wen-Hui,Wang, Ting-Yi,Yu, Huang-Ping,Hsieh, Pei-Wen
, p. 1123 - 1134 (2015/03/04)
Proteinase 3 (Pr3), and human neutrophil elastase (HNE) are two major neutrophilic serine proteases (NsPs) expressed in neutrophil azurophil granules. Emerging data suggest that excessive release of proteases mediates tissue damage, and therefore prolonged neutrophil accumulation has an important role in the pathogenesis of many diseases. Thus, HNE and Pr3 inhibitors may prove to be targets for the generation of agents in the treatment of neutrophilic inflammatory disease. Sivelestat is the only commercially available selective HNE inhibitor. Therefore, sivelestat was chosen as the model structure in an attempt to obtain more potent anti-NsPs agents. In the present study, a series 2-aminobenzaldehyde oxime and 2-aminobenzoate analogs were synthesized and their inhibitory effects on NsPs (CatG, Pr3, and HNE) were determined, respectively. The results of structure-activity relationships studies concluded that a hydroxyl oxime moiety plays an important role in ligand-enzyme affinity through hydrogen bonding. As compound 6 had more potency and showed dual inhibitory effects on NE and Pr3, both in vitro and in vivo experiments were carried out to evaluate its selectivity, effects in cell-based assays, and efficacy in models of inflammation and damage. Compound 6 had the potential to reduce paw edema induced by LPS and HNE, as well as acute lung injury, and may be approved as a candidate for the development of new agents in the treatment of neutrophilic inflammatory diseases.
Efficient synthesis of sivelestat sodium hydrate
Bijukumar,Maloyesh,Sampat,Bhirud,Rajendra
, p. 1718 - 1724 (2008/09/20)
An efficient and scaleable synthesis of sivelestat sodium hydrate has been developed. Copyright Taylor & Francis Group, LLC.
Non-peptidic inhibitors of human neutrophil elastase: The design and synthesis of sulfonanilide-containing inhibitors
Imaki, Katsuhiro,Okada, Takanori,Nakayama, Yoshisuke,Nagao, Yuuki,Kobayashi, Kaoru,Sakai, Yasuhiro,Mohri, Tetsuya,Amino, Takaaki,Nakai, Hisao,Kawamura, Masanori
, p. 2115 - 2134 (2007/10/03)
A novel series of pivaloyloxy benzene derivatives has been identified as potent and selective human neutrophil elastase (HNE) inhibitors. Convergent syntheses were developed in order to identify the inhibitors which are intravenously effective in an animal model. A compound of particular interest is the sulfonanilide-containing analogues. Structure-activity relationships are discussed. Structural requirements for metabolic stabilization are also discussed.
Derivatives of p-substituted phenyl ester of pivalic acid
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, (2008/06/13)
A derivative of general formula: STR1 wherein Y is --SO2 -- or STR2 (i) R1 and R2, which may be the same or different, each represent, (1) --H, (2) C1-16 alkyl or (3) the formula: --X-- A --(R4)n wher
