153097-60-0

Upstream product

• 97054-17-6 H-Gly-Leu-Val-NH₂ 
• 153097-58-6 Z-Arg(Z₂)-Leu-Leu-Gln-OH 
• 56-85-9 L-glutamine 
• 39802-26-1 Z-Leu-Gln-OH 
• 94770-42-0 H-Leu-Leu-Gln-OH 
• 52060-87-4 Z-Leu-Leu-Gln-OH 
• 41120-77-8 BOC-Gly-Leu-Val-NH₂ 
• 38062-70-3 H-Leu-Gln-OH 
• 3397-35-1 Z-L-leucine hydroxysuccinimide ester 
• 132160-73-7 Z-Arg(Z₂)-OSu 

Relevant academic research and scientific papers

Zur Totalsynthese von Human-Sekretin

The synthesis of the heptacosapeptide amide with the primary structure of Human-secretin is described.For this purpose 7 fragments were designed, i.e.H-Gly-Leu-Val-NH2 , Z-Arg(Z2)-Leu-Leu-Gln-OH , Z-Arg(Z2)-Leu-Gln-OH , Z-Arg(Z2)-Glu(OtBu)-Gly-Ala-OH , Z-Arg(Z2)-Leu-OH , Z-Thr(tBu)-Ser(tBu)-Glu(OtBu)-Leu-Ser(tBu)-OH , Adoc-His(Adoc)-Ser(tBu)-Asp(OtBu)-Gly-Thr(tBu)-Phe-OH ; these fragments were consequently assembled to the overall protected total sequence using the Wuensch/Weygand-method with dicyclohexylcarbodiimide.After deprotection by exposure to trifluoroacetic acid in presence of 1,2-ethanediol and water as scavenger, the islated crude product was purified by column chromatography on CM-Sepharose, fast flow.This synthesized Human-secretin showed the full biological activity in comparison to Porcine-secretin. Key words: Gastrointestinal hormone; Human-secretin: synthetic peptide factors.