154656-78-7

Upstream product

• 958-09-8 2'-deoxy-D-adenosine 
• 17331-12-3 N⁶-(dimethylamino-methylene)-2'-deoxy-adenosine 
• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 17331-20-3 5'-O-(4,4'-Dimethoxytrityl)-N⁶-dimethylformamidine-2'-deoxyadenosine 
• 102691-36-1 N,N,N',N'-tetraisopropyl 2-cyanoethylphosphorodiamidite 

Relevant academic research and scientific papers

FAST OLIGONUCLEOTIDE DEPROTECTION. PHOSPHORAMIDITE CHEMISTRY FOR DNA SYNTHESIS

A new set of base protecting groups for cyanoethylphosphoramidite nucleosides has been developed which decreases the post-synthesis time requirements.The traditional purine amide protecting groups, which require 8 - 16 hours at 55 deg C for deprotection in ammonia, have been replaced with the dimethylformamidine group.Oligonucleotides made with the new reagents require only 1 hour at 55 deg C or 8 hours at room temperature for complete deprotection.Dialkylformamidine phosphoramidites exhibit enhanced resistance to depurination compared to the traditional, or even the phenoxyacetyl, phosphoramidites.