FAST OLIGONUCLEOTIDE DEPROTECTION. PHOSPHORAMIDITE CHEMISTRY FOR DNA SYNTHESIS

Article abstract of DOI:10.1016/S0040-4039(00)88541-X

A new set of base protecting groups for cyanoethylphosphoramidite nucleosides has been developed which decreases the post-synthesis time requirements.The traditional purine amide protecting groups, which require 8 - 16 hours at 55 deg C for deprotection in ammonia, have been replaced with the dimethylformamidine group.Oligonucleotides made with the new reagents require only 1 hour at 55 deg C or 8 hours at room temperature for complete deprotection.Dialkylformamidine phosphoramidites exhibit enhanced resistance to depurination compared to the traditional, or even the phenoxyacetyl, phosphoramidites.