160054-76-2Relevant academic research and scientific papers
Influence of conformationally restricted pyrimidines on the activity of 10-23 DNAzymes
Robaldo, Laura,Izzo, Franco,Dellafiore, María,Proietti, Cecilia,Elizalde, Patricia V.,Montserrat, Javier M.,Iribarren, Adolfo M.
supporting information; scheme or table, p. 2581 - 2586 (2012/06/16)
The catalytic core of a 10-23 DNAzyme was modified introducing conformationally restricted nucleosides such as (2′R)-, (2′S)-2′-deoxy-2′-C-methyluridine, (2′R)-, (2′S)-2′-deoxy-2′-C-methylcytidine, 2,2′-anhydrouridine and LNA-C, in one, two or three positions. Catalytic activities under pseudo first order conditions were compared at different Mg2+ concentrations using a short RNA substrate. At low Mg2+ concentrations, triple modified DNAzymes with similar kinetic performance to that displayed by the non-modified control were identified. In the search for a partial explanation of the obtained results, in silico studies were carried out in order to explore the conformational behavior of 2′-deoxy-2′-C-methylpyrimidines in the context of a loop structure, suggesting that at least partial flexibility is needed for the maintenance of activity. Finally, the modified 2′-C-methyl DNAzyme activity was tested assessing the inhibition of Stat3 expression and the decrease in cell proliferation using the human breast cancer cell line T47D.
Synthesis of the phosphoramidite derivatives of 2′-deoxy-2′-C- α-methylcytidine and 2′deoxy-2′-C- αhydroxymethylcytidine: Analogues for chemical dissection of RNA's 2′-hydroxyl group
Li, Nan-Sheng,Piccirilli, Joseph A.
, p. 4751 - 4759 (2007/10/03)
Oligonucleotides containing 2′-C-αmethyl and 2′-C-α-hydroxymethyl modifications enable strategies for delineation of the distinctive role fulfilled by the 2′-hydroxyl group in RNA structure and function. Synthetic routes to the phosphoramidite derivatives
