1609534-55-5Relevant articles and documents
Combinatorially designed lipid-like nanoparticles for intracellular delivery of cytotoxic protein for cancer therapy
Wang, Ming,Alberti, Kyle,Sun, Shuo,Arellano, Carlos Luis,Xu, Qiaobing
, p. 2893 - 2898 (2014)
An efficient and safe method to deliver active proteins into the cytosol of targeted cells is highly desirable to advance protein-based therapeutics. A novel protein delivery platform has been created by combinatorial design of cationic lipid-like materials (termed lipidoids ), coupled with a reversible chemical protein engineering approach. Using ribonuclease A (RNase A) and saporin as two representative cytotoxic proteins, the combinatorial lipidoids efficiently deliver proteins into cancer cells and inhibit cell proliferation. A study of the structure-function relationship reveals that the electrostatic and hydrophobic interactions between the lipidoids and the protein play a vital role in the formation of protein-lipidoid nanocomplexes and intracellular delivery. A representative lipidoid (EC16-1) protein nanoparticle formulation inhibits cell proliferation in vitro and suppresses tumor growth in a murine breast cancer model.