161664-19-3Relevant academic research and scientific papers
REAGENTS FOR REVERSIBLY TERMINATING PRIMER EXTENSION
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Page/Page column 10-11, (2010/11/04)
This invention relates to the field of nucleic acid chemistry, more specifically to the field of compositions of matter that comprise triphosphates of modified 2'-deoxynucleosides and oligonucleotides that are formed when these are appended to the 3'-end of a primer, wherein said modifications comprise NH2 moiety attached to their 3'-hydroxyl group and a fluorescent species in a form of a tag affixed to the nucleobase via a linker that can be cleaved. Such compositions and their associated processes enable and improve the sequencing of oligonucleotides using a strategy of cyclic reversible termination, as outlined in US Patent 6,664,079. Most specifically, the invention concerns compositions of matter that are 5'-triphosphates of ribo- and 2'- deoxyribonucleosides carrying detectable tags and oligonucleotides that might be derived from them. The invention also concerns processes wherein a DNA polymerase, RNA polymerase, or reverse transcriptase synthesizes said oligonucleotides via addition of said triphosphates to a primer.
1-(2'-Deoxy-β-D-xylofuranosyl)cytosine: Base pairing of oligonucleotides with a configurationally altered sugar-phosphate backbone
Seela,Worner,Rosemeyer
, p. 883 - 896 (2007/10/02)
Solid-phase synthesis of the oligo(2'-deoxynucleotides) 19 and 20 containing 2'-deoxy-β-D-xylocytidine (4) is described. For this purpose, 1-(2-deoxy-β-D-threo-pentofuranosyl)cytosine (= 1-(2-deoxy-β-D-xylofuranosyl)cytosine; 4) was protected at its 4-NH
