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4-fluoro-N-(2,4,7-trinitrofluorenylidene)aniline, also known as 4-F-FNPA, is a chemical compound that serves as a substrate for certain enzymes, particularly esterases and lipases. It is a derivative of fluorescein, with a nitro group substitution pattern that enhances its reactivity and fluorescence properties. 4-fluoro-N-(2,4,7-trinitrofluorenylidene)aniline is widely used in biochemical research to study enzyme activity and inhibition, as well as in the development of biosensors. Upon enzymatic hydrolysis, 4-F-FNPA undergoes a color change from colorless to a highly fluorescent yellow, which can be easily detected and quantified. This property makes it a valuable tool for monitoring enzyme kinetics and for the detection of specific enzyme activities in complex biological samples.

1649-78-1

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1649-78-1 Usage

Check Digit Verification of cas no

The CAS Registry Mumber 1649-78-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,6,4 and 9 respectively; the second part has 2 digits, 7 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 1649-78:
(6*1)+(5*6)+(4*4)+(3*9)+(2*7)+(1*8)=101
101 % 10 = 1
So 1649-78-1 is a valid CAS Registry Number.

1649-78-1Downstream Products

1649-78-1Relevant academic research and scientific papers

Synthesis of N-(Nitrofluorenylidene)anilines and their Application as Electron Transport Materials in Positive Charge Electrophotography

Matsui, Masaki,Fukuyasu, Katsuyuki,Shibata, Katsuyoshi,Muramatsu, Hiroshige

, p. 1107 - 1110 (1993)

N-(Nitrofluorenylidene)anilines were synthesized by the condensation of nitrofluorenones with substituted anilines in the presence of zinc chloride. 2-Methyl, 2-ethyl, 2-isopropyl and 2-trifluoromethyl derivatives from 2,4,7-trinitrofluorenone showed high

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