16562-50-8 Usage
Uses
Used in Biochemical Assays:
4-nitrophenyl 3'-thymidylate is used as a substrate for enzymes in biochemical assays, allowing researchers to study the interactions and activities of enzymes such as thymidylate synthase, which plays a crucial role in DNA synthesis and repair.
Used in Molecular Biology and Biochemistry Experiments:
4-nitrophenyl 3'-thymidylate is used as a chromogenic or fluorescent label in molecular biology and biochemistry experiments. The 4-nitrophenyl group enables easy detection and quantification of the compound in a given sample, making it a valuable tool for researchers.
Used in Enzyme Kinetics Studies:
4-nitrophenyl 3'-thymidylate is used as a substrate in enzyme kinetics studies to investigate the catalytic properties and mechanisms of enzymes involved in DNA synthesis and repair.
Used in DNA Replication Research:
4-nitrophenyl 3'-thymidylate is used in research to study the process of DNA replication, providing insights into the molecular mechanisms underlying this essential biological process.
Check Digit Verification of cas no
The CAS Registry Mumber 16562-50-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,6,5,6 and 2 respectively; the second part has 2 digits, 5 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 16562-50:
(7*1)+(6*6)+(5*5)+(4*6)+(3*2)+(2*5)+(1*0)=108
108 % 10 = 8
So 16562-50-8 is a valid CAS Registry Number.
16562-50-8Relevant academic research and scientific papers
Synthesis of 4-Nitrophenyl Esters of Thymidine 3'-Phosphate and 3'-Phosphorothioate using a New Phosphorylating Agent
Niewiarowski, Wojcieh,Stec, Wojcieh J.,Zielinski, Wojcieh S.
, p. 524 - 525 (2007/10/02)
2'-Desoxynucleoside-3'-O-(4-nitrophenyl) phosphoranilidates (e.g., 2), prepared by the action of O-(4-nitrophenyl) N-phenylphosphoramidochloridate (1) on alcohols, are converted into the corresponding 4-nitrophenyl phosphate esters (e.g., 3) or P-chiral 4-nitrophenyl phosphorothioate esters (e.g., 4) by treatment with NaH-CX2 (X = O or S).
