17059-16-4Relevant articles and documents
Biotransformation of digitoxigenin by cultured Strophanthus hybrid cells
Kawaguchi, Kiichiro,Koike, Sachiko,Hirotani, Masao,Fujihara, Michio,Furuya, Tsutomu,Iwata, Riko,Morimoto, Kazuo
, p. 1261 - 1265 (2007/10/03)
Hybrid cells between Strophanthus gratus and S. amboensis were obtained by electrofusion and confirmed to be hybrids through isozyme and RFLP analyses. Because a new and hybrid compound, 17βH-periplogenin β-D- glucoside, was isolated as a biotransformation product of digitoxigenin by the hybrid cells, isomerization of 17β-lactone ring on S. gratus and glucosylation on S. amboensis were demonstrated simultaneously as the biotransformation abilities in the hybrid cells. Moreover, the productivity of the hybrid compound was increased by raising the sucrose concentration.
BIOTRANSFORMATION OF DIGITOXIGENIN BY GINSENG HAIRY ROOT CULTURES
Kawaguchi, Kiichiro,Hirotani, Masao,Yoshikawa, Takafumi,Furuya, Tsutomu
, p. 837 - 843 (2007/10/02)
Five new compounds (three esters and two glycosides) and seven previously reported compounds were isolated as biotransformation products of digitoxigenin by ginseng hairy root cultures.The new esters and glycosides were elucidated as digitoxigenin stearate, digitoxigenin palmitate, digitoxigenin myristate, 3-epidigitoxigenin β-D-gentiobioside and digitoxigenin β-D-sophoroside using 1H and 13C NMR and FAB mass spectral data.Biotransformations involving esterification (of stearic acid, palmitic acid, myristic acid and lauric acid) and glycosylation (of gentiobiose and sophorose) of digitoxigenin have been demonstrated for the first time in the plant cell and tissue cultures.The hairy roots showed high glycosylation ability to the digitoxigenin molecule.
BIOTRANSFORMATION OF DIGITOXIGENIN BY CELL SUSPENSION CULTURES OF DIGITALIS PURPUREA
Hirotani, Masao,Furuya, Tsutomu
, p. 531 - 534 (2007/10/02)
Digitoxigenin was oxidized to digitoxigenone which was reduced to epidigitoxigenin and then glucosylated to epidigitoxigenin glucoside by cell cultures of Digitalis purpurea.The epidigitoxigenin glucoside, together with digitoxigenone and epidigitoxigenin, was isolated in considerable amounts, whereas digitoxigenin glucoside could only be detected in low concentration.Furthermore, it was confirmed by TLC and HPLC that digitoxigenin was hydroxylated to periplogenin.Key Word Index-Digitalis purpurea; Scrophulariaceae; tissue cultures; biotransformation; hydroxylation; glucosylation; steroid; digitoxigenin; digitoxigenone; epidigitoxigenin; periplogenin; digitoxigenin glucoside; epidigitoxigenin glucoside.