17331-52-1Relevant academic research and scientific papers
Synthesis of 5-methyl-2'-O-deoxycytidine analogs to determine monoclonal antibody specificity in the recognition of the 6-(p-bromobenzoylamino) caproyl radical
Rodriguez-Tanty,Higginson-Clarke,Hernandez,Perez,Velez- Castro,Riveron,Macias
, p. 455 - 467 (1997)
Eight new p-bromobenzoyl derivatives of 5-methyl-2'-O-deoxycytidine analogs, substituted at the 4-position, were synthesized. The best conditions for obtaining 5-methyl-4-N-aminoalkyl-2'-O-deoxycytidine from 3',5'-di-O- acetyl-4-(1,2,4-triazol-1-yl)-2'-O-deoxythymidine were studied. The nucleoside analogs were used to identify the fragment of the 6-(p- bromobenzoylamino)caproyl radical that binds to the monoclonal antibody obtained against it and to define an affinity scale of monoclonal antibody against them.
Exploring the potential of 3′-O-carboxy esters of thymidine as inhibitors of ribonuclease A and angiogenin
Ghosh, Kalyan S.,Debnath, Joy,Dutta, Palash,Sahoo, Bijaya K.,Dasgupta, Swagata
, p. 2819 - 2828 (2008)
In this study, compounds with a carboxy ester in lieu of the phosphate ester at the 3′-position have been employed to inhibit the ribonucleolytic activity of ribonuclease A (RNase A). Phosphates at the 3′-position of pyrimidine bases are well-known inhibitors of the protein. We have investigated the inhibition of RNase A by 3′-O-carboxy esters of thymidine. The compounds behave as competitive inhibitors with inhibition constants ranging from 42 to 95 μM. The mode of inhibition has also been confirmed by 1H NMR studies of the active site histidines of RNase A. Docking studies have further substantiated the experimental results. The compounds are also found to inhibit the ribonucleolytic activity of angiogenin, a homologous protein and potent inducer of blood vessel formation.
