174571-56-3

Upstream product

• 174571-54-1 3'-azido-3'-deoxy-5'-O-(4-methoxybenzoyl)-N³-{1-[5-(2,2,2,-trifluoroacetylamino)pentyl]}thymidine 
• 128238-44-8 2,2,2-trifluoro-N-(5-hydroxypentyl)acetamide 
• 134077-32-0 3'-Azido-5'-O-(4-methoxybenzoyl)-3'-deoxythymidine 
• 165271-67-0 <<(Benzyloxy)<<(benzyloxy)hydroxyphosphoryl>methyl>phosphoryl>methyl>phosphonic acid dibenzyl ester 
• 174571-55-2 3'-azido-3'-deoxy-N³-{1-[5-(2,2,2-trifluoroacetylamino)pentyl]}thymidine 

Relevant academic research and scientific papers

Synthesis of Polyphosphorylated AZT Derivatives for the Development of Specific Enzyme Immunoassays

The synthesis of a series of analogues of the different polyphosphorylated metabolites of AZT has been carried out. The compounds were designed in order to raise specific antibodies for the development of highly sensitive titration kits for the intracellular metabolites of AZT. The pyrophosphate moiety in AZT-DP and AZT-TP analogues is mimicked by the methylene bisphosphonate group to provide in vivo stability of the compounds. An ω-amino linker was introduced at the N3 position on the base to allow the further anchoring of the compounds to a carrier protein before immunization, tentitatively focusing the specificity of the immune response toward the phosphate mimic moiety and the nonnatural sugar.

Synthesis of a non-hydrolyzable AZT-triphosphate analogue designed for the production of anti-AZT-TP antibodies

An enzymatically stable analogue of AZT-triphosphate is prepared in 7 steps starting from thymidine. The thymine moiety is functionalized at N3 to allow the further cross-linking to a carrier protein for subsequent immunization of rabbits in order to raise specific antibodies directed against AZT-TP.