17833-43-1 Usage
Description
4-Methylumbelliferyl-α-D-glucopyranoside is a fluorogenic substrate for α-glucosidase. 4-Methylumbelliferyl-α-D-glucopyranoside is cleaved by α-glucosidase to release the fluorescent moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high pH (7.12-10.3), respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. 4-Methylumbelliferyl-α-D-glucopyranoside has been used to quantify α-glucosidase activity in infant blood spot samples as a biomarker of Fabry and Pompe diseases, lysosomal storage disorders characterized by a deficiency in the enzyme.
Chemical Properties
white to off-white crystalline powder
Uses
Different sources of media describe the Uses of 17833-43-1 differently. You can refer to the following data:
1. 4-Methylumbelliferyl-α-D-glucopyranoside is a fluorogenic substrate in the assay of a-glucosidase,it has been used in the study of Pompe disease.
2. 4-Methylumbelliferyl α-D-glucopyranoside is used as a flurogenic substrate for the identification, characterization and kinetic analysis of α-D-glucosidase(s).
Purification Methods
Recrystallise 4-methylumbellifer-7-yl--D-glucopyranoside from hot H2O or EtOH. [Courtin-Duchateau & Veyrière Carbohydr Research 65 23, 29 1978, Beilstein 18 IV 443.]
Check Digit Verification of cas no
The CAS Registry Mumber 17833-43-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,7,8,3 and 3 respectively; the second part has 2 digits, 4 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 17833-43:
(7*1)+(6*7)+(5*8)+(4*3)+(3*3)+(2*4)+(1*3)=121
121 % 10 = 1
So 17833-43-1 is a valid CAS Registry Number.
InChI:InChI=1/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16+/m0/s1
17833-43-1Relevant articles and documents
Concise synthesis of sulfoquinovose and sulfoquinovosyl diacylglycerides, and development of a fluorogenic substrate for sulfoquinovosidases
Zhang, Yunyang,Mui, Janice W.-Y.,Arumaperuma, Thimali,Lingford, James P.,Goddard-Borger, Ethan D.,White, Jonathan M.,Williams, Spencer J.
, p. 675 - 686 (2020)
The sulfolipid sulfoquinovosyl diacylglycerol (SQDG) and its headgroup, the sulfosugar sulfoquinovose (SQ), are estimated to harbour up to half of all organosulfur in the biosphere. SQ is liberated from SQDG and related glycosides by the action of sulfoquinovosidases (SQases). We report a 10-step synthesis of SQDG that we apply to the preparation of saturated and unsaturated lipoforms. We also report an expeditious synthesis of SQ and (13C6)SQ, and X-ray crystal structures of sodium and potassium salts of SQ. Finally, we report the synthesis of a fluorogenic SQase substrate, methylumbelliferyl α-d-sulfoquinovoside, and examination of its cleavage kinetics by two recombinant SQases. These compounds will assist in dissecting the role of sulfoglycolysis in the biogeochemical sulfur cycle and understanding the molecular basis of sulfoglycolysis.
