179178-43-9Relevant articles and documents
Tuning single nucleotide discrimination in polymerase chain reactions (PCRs): Synthesis of primer probes bearing polar 4′-C-modifications and their application in allele-specific PCR
Gaster, Jens,Marx, Andreas
, p. 1861 - 1870 (2007/10/03)
There are many methods available for the detection of nucleotide variations in genetic material. Most of these methods are applied after amplification of the target genome sequence by the polymerase chain reaction (PCR). Many efforts are currently underwa
Sugar modified nucleosides and their use for synthesis of oligonucleotides
-
, (2008/06/13)
A number of modified nucleosides are disclosed composed of modified sugar moieties which contain substituents at C1 and C4 positions, or branched substituents at C3 and C5 positions of deoxyribose or ribose. Each nucleoside is converted to or properly pro
Synthesis and evaluation of oligodeoxynucleotides containing 4'-C-substituted thymidines
Wang, Guangyi,Seifert, Wilfried E.
, p. 6515 - 6518 (2007/10/03)
4'-C-Hydroxymethylthymidine was converted to 4'-C-methoxymethylthymidine and 4'-C-aminomethylthymidine, which were incorporated into oligodeoxynucleotides by phosphoramidite chemistry. The modified oligonucleotides exhibit excellent hybridization and significant improvement in stability to snake venom phosphodiesterase.