184229-73-0Relevant academic research and scientific papers
Efficient solid phase synthesis of cleavable oligodeoxynucleotides based on a novel strategy for the synthesis of 5′-S-(4,4′-dimethoxytrityl)- 2′-deoxy-5′-thionucleoside phosphoramidites
Jahn-Hofmann, Kerstin,Engels, Joachim W.
, p. 2812 - 2828 (2007/10/03)
The incorporation of a specific cleavage site into an oligodeoxynucleotide can be achieved by utilizing the four 5′-S-(4,4′-dimethoxytrityl)- 2′-deoxy-5′-thionucleoside 3′-(2-cyanoethyl diisopropylphosphoramidites) 5 and 15a-c (Fig.1). Based on the silver
A new concept for DNA-arrays
Jahn-Hofmann, Kerstin,Holzhey, Nancy,Ellinger, Thomas,Engels, Joachim W.
, p. 1479 - 1482 (2007/10/03)
We will insert a cleavage site in an oligodeoxynucleotide, which can be used for a selective and quantitative cleavage. For that reason we synthesized the four 5′-S-(4,4′-dimethoxytrityl)-mercapto-2′ -deoxynucleotide-3′-O-(2-cyanoethoxydiisopropylamino)-p
Alteration of DNA primary structure by DNA topoisomerase I. Isolation of the covalent topoisomerase I-DNA binary complex in enzymatically competent form
Henningfeld, Kristine A.,Arslan, Tuncer,Hecht, Sidney M.
, p. 11701 - 11714 (2007/10/03)
DNA ligation by DNA topoisomerase I was investigated employing synthetic DNA substrates containing a single strand nick. Site-specific cleavage of the DNA by topoisomerase I in proximity to the nick resulted in uncoupling of the cleavage and ligation reac
