186801-75-2Relevant academic research and scientific papers
Simultaneous quantification of multiple nucleic acid targets using chemiluminescent probes
Browne, Kenneth A.,Deheyn, Dimitri D.,El-Hiti, Gamal A.,Smith, Keith,Weeks, Ian
, p. 14637 - 14648 (2011)
A novel method is described for simultaneous detection and quantification of attomoles or a few femtomoles of two (or potentially more) nucleic acid targets, without need for amplification. The technique depends on spectral-temporal resolution of chemiluminescence emitted from independent hybridization-induced chemiluminescent signal probes. The probes are internally quenched except in the presence of their specific targets, thereby allowing detection limits up to 10 000 times lower than with fluorescent probes. This is sufficient to obviate the need for amplification in many cases. The utility of the technique has been demonstrated by use of resolvable N-linked acridinium and 2,7-dimethoxyacridinium ester labeled probes in a homogeneous assay for sensitive and simultaneous independent quantification of pan-bacterial and pan-fungal target sequences in seawater.
Chemiluminescence of methoxycarbonylphenyl 10-methyl-10λ4 -2,7-disubstituted acridine-9-carboxylate derivatives
Nakazono, Manabu,Nanbu, Shinkoh,Akita, Takeyuki,Hamase, Kenji
, (2020)
Methoxycarbonylphenyl 10-methyl-10λ4-2,7-disubstituted acridine-9-carboxylate derivatives were synthesized, and both their chemiluminescence intensities at pH 7?10 and their chemical stabilities were clarified. The products and generation efficiency of the chemiluminescence reactions were evaluated. 4-(Methoxycarbonyl)phenyl 10-methyl-10λ4-2,7-dimethylacridine-9-carboxylate gave relatively strong chemiluminescence intensities at pH 7?10 and was applied to the measurement of hydrogen peroxide.
CHEMILUMINESCENT PROBES FOR MULTIPLEX MOLECULAR QUANTIFICATION AND USES THEREOF
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, (2012/09/22)
A novel method is disclosed for simultaneous detection and quantification of two or more nucleic acid targets, without need for amplification. The method depends on spectral-temporal resolution of chemiluminescence emitted from independent hybridization-induced chemiluminescent signal (HICS) probes. The utility of this method has been demonstrated by use of resolvable N-linked acridinium and 2,7-dimethoxyacridinium ester labeled probes in a homogeneous assay for sensitive and simultaneous independent quantification of several bacterial and fungal target sequences. Compositions and kits for practicing the method of the present invention are also disclosed.
