187139-97-5Relevant articles and documents
Synthesis of octyl O- and S-glycosides related to the GPI anchor of Trypanosoma brucei and their in vitro galactosylation by trypanosomal α-galactosyltransferases
Ziegler, Thomas,Dettmann, Ralf,Duszenko, Michael,Kolb, Volker
, p. 7 - 23 (2007/10/03)
Octyl O- and S-glycosides of mono- to tri-saccharides related to the core structure α-D-Manp-(1→2)-α-D-Manp-(1→6)-α-D-Manp of the GPI anchor of Trypanosoma brucei have been prepared via regioselective protodesilylation and glycodesilylation of octyl O- and S-glycosides of 2-O-benzoyl-4,6-O-(1,1,3,3-tetraisopropyl-1,3-disiloxane-1,3-diyl)-α-D -mannopyranoside. The synthetic saccharides have been used as substrates for enzymatic α-galactosylation with membrane fractions of bloodstream forms of T. brucei strain 427 variants MITat 1.4, MITat 1.2, and MITat 1.5, respectively.