188411-06-5 Usage
Uses
Used in DNA Synthesis:
N4-benzoyl-5-(2-cyanoethyl)hydroxymethyl-5'-(4,4'-dimethoxytrityl)-2'-deoxycytidine-3'-[N,N-diisopropyl-N-cyanoethyl]phosphoramidite is used as a protecting reagent and phosphorylating agent for the synthesis of DNA. It helps in the formation of DNA strands by protecting the nucleotide building blocks and activating them for the subsequent steps in the synthesis process.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, N4-benzoyl-5-(2-cyanoethyl)hydroxymethyl-5'-(4,4'-dimethoxytrityl)-2'-deoxycytidine-3'-[N,N-diisopropyl-N-cyanoethyl]phosphoramidite is used as a key component in the development of drugs targeting genetic disorders and diseases. Its role in DNA synthesis allows for the creation of specific DNA sequences that can be used in the design and testing of new therapeutic agents.
Used in Research and Development:
N4-benzoyl-5-(2-cyanoethyl)hydroxymethyl-5'-(4,4'-dimethoxytrityl)-2'-deoxycytidine-3'-[N,N-diisopropyl-N-cyanoethyl]phosphoramidite is also utilized in research and development for studying the structure, function, and interactions of DNA molecules. Its applications in this field contribute to a better understanding of genetic information and the development of new techniques for genetic analysis and manipulation.
Check Digit Verification of cas no
The CAS Registry Mumber 188411-06-5 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,8,8,4,1 and 1 respectively; the second part has 2 digits, 0 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 188411-06:
(8*1)+(7*8)+(6*8)+(5*4)+(4*1)+(3*1)+(2*0)+(1*6)=145
145 % 10 = 5
So 188411-06-5 is a valid CAS Registry Number.
188411-06-5Relevant articles and documents
Improved synthesis of 5-hydroxymethyl-2′-deoxycytidine phosphoramidite using a 2′-deoxyuridine to 2′-deoxycytidine conversion without temporary protecting groups
Hansen, Anders S.,Thalhammer, Armin,El-Sagheer, Afaf H.,Brown, Tom,Schofield, Christopher J.
, p. 1181 - 1184 (2011/04/16)
5-Hydroxymethylcytosine has recently been characterized as the 'sixth base' in human DNA. To enable research on this DNA modification, we report an improved method for the synthesis of 5-hydroxymethyl-2′-deoxycytidine (5-HOMedC) phosphoramidite for site-specific incorporation into oligonucleotides. To minimize manipulations we employed a temporary protecting group-free 2′-deoxyuridine to 2′-deoxycytidine conversion procedure that utilizes phase transfer catalysis. The desired 5-HOMedC phosphoramidite is obtained in six steps and 24% overall yield from 2′-deoxyuridine.
Hydroxylation of methylated CpG dinucleotides reverses stabilisation of DNA duplexes by cytosine 5-methylation
Thalhammer, Armin,Hansen, Anders S.,El-Sagheer, Afaf H.,Brown, Tom,Schofield, Christopher J.
, p. 5325 - 5327 (2011/06/21)
Cytosine-5-methylation stabilises DNA duplexes and is associated with transcriptional repression; 5-methylcytosine undergoes hydroxylation to 5-hydroxymethylcytosine, a modification of unknown biological function. Spectroscopic and calorimetric analyses show that 5-hydroxymethylcytosine introduction reverses the stabilising effect of 5-methylcytosine, suggesting that in some contexts, 5-methylcytosine hydroxylation may, along with other factors, contribute to the alleviation of transcriptional repression.
