188712-78-9Relevant academic research and scientific papers
Benzophenoxazine dyes
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, (2008/06/13)
PCT No. PCT/GB97/00324 Sec. 371 Date Jan. 15, 1999 Sec. 102(e) Date Jan. 15, 1999 PCT Filed Feb. 5, 1997 PCT Pub. No. WO97/29154 PCT Pub. Date Aug. 14, 1997Benzophenoxazine compounds have formula (I) where X is O or NH, Y is NR1R2 or H, R1 and R2 are alkyl or -L-A, L is a linker and A may be a reactive group by means of which the compound is linked to a biomolecule. The compounds can be used as fluorescent dyes for labelling biomolecules.
Synthesis of functionalised fluorescent dyes and their coupling to amines and amino acids
Briggs, Mark S. J.,Bruce, Ian,Miller, James N.,Moody, Christopher J.,Simmonds, Adrian C.,Swann, Elizabeth
, p. 1051 - 1058 (2007/10/03)
A series of novel functionalised benzophenoxazinones, analogues of Nile Red, is prepared and their fluorescence properties evaluated. The ring system is prepared by reaction of 5-diethylamino-2-nitrosophenol with 1,6-dihydroxynaphthalene followed by alkylation of the 2-hydroxy group with 6-bromohexanoic acid derivatives. Subsequent ester cleavage under a variety of conditions gives the acid 6. Replacement of the 9-diethylamino group with the N-ethyl-(3-sulfonylpropyl)amino group is carried out to increase water solubility and the resulting dye 14 has similar fluorescence properties. The acid 6 is coupled to a range of amino compounds.
