192374-17-7

Basic information

• Product Name: 6-CARBOXYFLUORESCEIN DIPIVALATE
• Synonyms: 6-CARBOXYFLUORESCEIN DIPIVALATE;6-CARBOXYFLUORESCEIN-PIV;Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid, 3',6'-bis(2,2-dimethyl-1-oxopropoxy)-3-oxo-;6-carboxy-3',6'-di-O-pivaloylfluorescein, 5-carboxyfluorescein dipivalate, 6-carboxyfluorescein-3',6'-dipivalat, 6-carboxyfluorescein dipivaloate, 6-carboxyfluorescein dipivalate
• CAS NO: 192374-17-7
• Molecular Formula: C₃₁H₂₈O₉
• Molecular Weight: 544.55
• Mol File: 192374-17-7.mol
• Article Data: 4

Chemical Properties

• Appearance: /
• CAS DataBase Reference: 6-CARBOXYFLUORESCEIN DIPIVALATE(CAS DataBase Reference)
• NIST Chemistry Reference: 6-CARBOXYFLUORESCEIN DIPIVALATE(192374-17-7)
• EPA Substance Registry System: 6-CARBOXYFLUORESCEIN DIPIVALATE(192374-17-7)

Safety Data

• Hazardous Substances Data: 192374-17-7(Hazardous Substances Data)

Usage

Uses

3'',6''-Bis(2,2-dimethyl-1-oxopropoxy)-3-oxospiro[isobenzofuran-1(3H),9''-[9H]xanthene]-6-carboxylic Acid is used as fluorescent tracer. It was used as a reactant in the specific molecular beacon probe for the detection of human prostate cancer cells.

Upstream product

• 847569-43-1 C₃₁H₂₈O₉*C₆H₁₅N 
• 108-46-3 recorcinol 
• 552-30-7 trimellitic Anhydride 
• 1538-75-6 2,2-dimethylpropanoic anhydride 
• 3301-79-9 6-carboxyfluorescein 

Downstream Products

• 1275574-82-7 6-carboxyfluorescein-β-Ala-O-DMT-serinol 
• 637330-25-7 5'-O-(4,4'-dimethoxytrityl)-5-{6-(dipivaloyl-5-carboxyfluoresceinamido)caproamidoprop-2-ynyl}-2'-deoxyuridine 
• 197850-75-2 1-{[(3,6-di-O-pivaloyl)fluorescein-6-yl]oxy}pyrrolidine-2,5-dione 
• 1381804-09-6 C₃₁H₂₁NO₇ 
• 478801-49-9 N-(2''-propynyl)-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-6-carboxamide 

Relevant articles and documents

Optical Manipulation of Subcellular Protein Translocation Using a Photoactivatable Covalent Labeling System

The photoactivatable chemically induced dimerization (photo-CID) technique for tag-fused proteins is one of the most promising methods for regulating subcellular protein translocations and protein–protein interactions. However, light-induced covalent protein dimerization in living cells has yet to be established, despite its various advantages. Herein, we developed a photoactivatable covalent protein-labeling technology by applying a caged ligand to the BL-tag system, a covalent protein labeling system that uses mutant β-lactamase. We further developed CBHD, a caged protein dimerizer, using caged BL-tag and HaloTag ligands, and achieved light-induced protein translocation from the cytoplasm to subcellular regions. In addition, this covalent photo-CID system enabled quick protein translocation to a laser-illuminated microregion. These results indicate that the covalent photo-CID system will expand the scope of CID applications in the optical manipulation of cellular functions.

POLYMERASE-INDEPENDENT ANALYSIS OF THE SEQUENCE OF POLYNUCLEOTIDES

The present invention concerns methods of polymerase independent template directed elongation of polynucleotides, nucleotide building blocks used in these methods as well as the use of the methods and building blocks for the determination of nucleotide sequences, in particular for the determination of SNPs, base modifications, mutations, rearrangements and methylation patterns.