478801-49-9

Usage

Uses

Used in Bioconjugation:
FAM alkyne, 6-isomer is used as a bioconjugation agent for attaching biomolecules such as proteins, peptides, and nucleic acids to other molecules or surfaces. The alkyne group enables copper-catalyzed Click chemistry, allowing for efficient and specific conjugation.
Used in Fluorescence Imaging:
FAM alkyne, 6-isomer is used as a fluorescent probe for imaging biological processes in vitro and in vivo. Its high aqueous solubility and fluorescence properties make it suitable for visualizing cellular structures, tracking molecular interactions, and monitoring biological events.
Used in Drug Discovery:
FAM alkyne, 6-isomer is used as a tool in drug discovery for labeling and tracking drug candidates. Its fluorescence allows for the detection and quantification of drug molecules in biological systems, aiding in the evaluation of drug efficacy and safety.
Used in Chemical Biology:
FAM alkyne, 6-isomer is used as a building block in the development of chemical probes and sensors. Its alkyne functionality enables copper-catalyzed Click chemistry, allowing for the rapid assembly of complex molecular structures and the creation of novel probes for studying biological systems.

Upstream product

• 847569-43-1 C₃₁H₂₈O₉*C₆H₁₅N 
• 108-46-3 recorcinol 
• 552-30-7 trimellitic Anhydride 
• 192374-17-7 3',6'-bis(2,2-dimethyl-1-oxopropoxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid 
• 1381804-22-3 N-(2''-propynyl)-3',6'-dipivaloyloxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-6-carboxamide 

Downstream Products

• 1381804-09-6 C₃₁H₂₁NO₇ 
• 4238-39-5 N-[4-[(1E)-2-[4-(dimethylamino)phenyl]diazenyl]benzoyl]-glycine 

Relevant academic research and scientific papers

Optical Manipulation of Subcellular Protein Translocation Using a Photoactivatable Covalent Labeling System

The photoactivatable chemically induced dimerization (photo-CID) technique for tag-fused proteins is one of the most promising methods for regulating subcellular protein translocations and protein–protein interactions. However, light-induced covalent protein dimerization in living cells has yet to be established, despite its various advantages. Herein, we developed a photoactivatable covalent protein-labeling technology by applying a caged ligand to the BL-tag system, a covalent protein labeling system that uses mutant β-lactamase. We further developed CBHD, a caged protein dimerizer, using caged BL-tag and HaloTag ligands, and achieved light-induced protein translocation from the cytoplasm to subcellular regions. In addition, this covalent photo-CID system enabled quick protein translocation to a laser-illuminated microregion. These results indicate that the covalent photo-CID system will expand the scope of CID applications in the optical manipulation of cellular functions.

A specific molecular beacon probe for the detection of human prostate cancer cells

The small-molecule, water-soluble molecular beacon probe 1 is hydrolyzed by the lysate and living cells of human prostate cancer cell lines (LNCaP), resulting in strong green fluorescence. In contrast, probe 1 does not undergo significant hydrolysis in ei