197850-75-2

Basic information

• Product Name: 5,6-CARBOXYFLUORESCEIN DIPIVALATE SUCCINIMIDE ESTER
• Synonyms: 6-CARBOXYFLUORESCEIN-PIV SE;6-CARBOXYFLUORESCEIN DIPIVALATE-N-HYDROXYSUCCINIMIDE;6-CARBOXYFLUORESCEIN DIPIVALATE SUCCINIMIDE ESTER;5,6-CARBOXYFLUORESCEIN DIPIVALATE SUCCINIMIDE ESTER;5,6-CARBOXYFLUORESCEIN-PIV SE;6-CARBOXYFLUORESCEIN DIPIVALATE NHS ESTER;6-CARBOXYFLUORESCEIN DIPIVALATE N-HYDROXYSUCCINIMIDE ESTER
• CAS NO: 197850-75-2
• Molecular Formula: C₃₅H₃₁NO₁₁
• Molecular Weight: 641.62
• Mol File: 197850-75-2.mol
• Article Data: 4

Chemical Properties

• Appearance: /
• CAS DataBase Reference: 5,6-CARBOXYFLUORESCEIN DIPIVALATE SUCCINIMIDE ESTER(CAS DataBase Reference)
• NIST Chemistry Reference: 5,6-CARBOXYFLUORESCEIN DIPIVALATE SUCCINIMIDE ESTER(197850-75-2)
• EPA Substance Registry System: 5,6-CARBOXYFLUORESCEIN DIPIVALATE SUCCINIMIDE ESTER(197850-75-2)

Safety Data

• Hazardous Substances Data: 197850-75-2(Hazardous Substances Data)

Usage

Uses

6-Carboxyfluorescein Dipivalate N-Hydroxysuccinimide Ester is used as a probe in fluorescence activation-coupled protein labeling method for real-time imaging/measurements of protein dynamics.

Upstream product

• 6066-82-6 1-hydroxy-pyrrolidine-2,5-dione 
• 192374-17-7 3',6'-bis(2,2-dimethyl-1-oxopropoxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid 
• 3301-79-9 6-carboxyfluorescein 
• 1538-75-6 2,2-dimethylpropanoic anhydride 

Downstream Products

• 866824-20-6 N-(3',6'-dipivaloylfluorescein-6-ylcarbonyl)-3'-amino-3'-deoxythymidine 

Relevant articles and documents

Fluorescent mimetics of CMP-Neu5Ac are highly potent, cell-permeable polarization probes of eukaryotic and bacterial sialyltransferases and inhibit cellular sialylation

Oligosaccharides of the glycolipids and glycoproteins at the outer membranes of human cells carry terminal neuraminic acids, which are responsible for recognition events and adhesion of cells, bacteria, and virus particles. The synthesis of neuraminic acid containing glycosides is accomplished by intracellular sialyl transferases. Therefore, the chemical manipulation of cellular sialylation could be very important to interfere with cancer development, inflammations, and infections. The development and applications of the first nanomolar fluorescent inhibitors of sialyl transferases are described herein. The obtained carbohydrate-nucleotide mimetics were found to bind all four commercially available and tested eukaryotic and bacterial sialyl transferases in a fluorescence polarization assay. Moreover, it was observed that the anionic mimetics intruded rapidly and efficiently into cells in vesicles and translocated to cellular organelles surrounding the nucleus of CHO cells. The new compounds inhibit cellular sialylation in two cell lines and open new perspectives for investigations of cellular sialylation. You can't run, you can't hide: Sialyltransferases cover cancer cells with neuraminic acids, enabling them to escape from tissues and to metastasize. Cell-permeable chemical probes targeting this class of enzymes might help to study, understand, and inhibit such processes.

POLYMERASE-INDEPENDENT ANALYSIS OF THE SEQUENCE OF POLYNUCLEOTIDES

The present invention concerns methods of polymerase independent template directed elongation of polynucleotides, nucleotide building blocks used in these methods as well as the use of the methods and building blocks for the determination of nucleotide sequences, in particular for the determination of SNPs, base modifications, mutations, rearrangements and methylation patterns.