193967-37-2Relevant articles and documents
A new route to oligodeoxynucleoside phosphoramidates (P-NH2)
Laurent, Alain,Debart, Francoise,Rayner, Bernard
, p. 5285 - 5288 (2007/10/03)
We describe a new strategy to introduce phosphoramidate (P-NH2) internucleosidic linkages in oligonucleoside phosphodiesters using dimer building blocks. The P-NH2 function is generated under photolysis of the N-2-nitrobenzylphosphoramidate linkage (P-NHR). We achieved the synthesis of N-2-nitrobenzylphosphoramidate β and α-anomeric nucleotides dimers via either phosphoramidite or H-phosphonate chemistry, α-dimer (P-NHR) was incorporated at defined positions into α-oligonucleoside phosphodiesters and the P-NH2 linkage was liberated by U.V irradiation.