194785-18-7

Usage

Uses

Used in Research and Diagnostics:
6-Carboxy-X-Rhodamine is used as a tracer agent for tracking and visualizing the movement, distribution, and localization of molecules or cells within biological systems. Its fluorescent properties allow for easy detection and monitoring, making it a valuable tool in research and diagnostics.
Used in Medical Applications:
In the medical field, 6-Carboxy-X-Rhodamine is used as a labeling agent for biologics, such as proteins, antibodies, and nucleic acids. This enables the identification, quantification, and study of these biomolecules, contributing to the development of new drugs and therapies.
Used in Laser Technology:
6-Carboxy-X-Rhodamine is also utilized as a laser dye in various laser applications due to its ability to efficiently convert light energy into specific wavelengths. This makes it suitable for use in laser systems that require precise control over the emitted light, such as in medical procedures, scientific research, and industrial processes.
Used in Analytical Chemistry:
In analytical chemistry, 6-Carboxy-X-Rhodamine serves as a valuable tool for the detection and quantification of specific substances. Its fluorescent properties allow for sensitive and selective detection, making it an essential component in the development of new analytical methods and techniques.
Used in Material Science:
6-Carboxy-X-Rhodamine is employed in the development of advanced materials with specific optical properties. Its ability to emit light at specific wavelengths makes it a useful component in the creation of materials for applications such as optical sensors, display technologies, and photovoltaic devices.
Used in Environmental Monitoring:
In environmental monitoring, 6-Carboxy-X-Rhodamine is used as a tracer to study the behavior and fate of pollutants in the environment. Its fluorescent properties enable the detection and tracking of contaminants, helping to assess their impact on ecosystems and inform strategies for pollution control and remediation.

InChI:InChI=1/C33H30N2O5/c36-32(37)20-9-10-21(33(38)39)24(17-20)27-25-15-18-5-1-11-34-13-3-7-22(28(18)34)30(25)40-31-23-8-4-14-35-12-2-6-19(29(23)35)16-26(27)31/h9-10,15-17H,1-8,11-14H2,(H-,36,37,38,39)

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Relevant academic research and scientific papers

Preparation process of carboxyl-X-rhodamine

The invention provides a preparation process of carboxyl-X-rhodamine, which adopts 8-hydroxyjulolidine phenol and trimellitic anhydride as raw materials, an intermediate 5, 6-carboxyl-X-rhodamine is synthesized by a direct cyclization method, a high-boiling-point solvent is adopted as a cyclization medium, and a cyclization product has poor solubility in the solvent and can be precipitated in a reaction system, so that the yield of 5, 6-carboxyl-X-rhodamine is increased, and the yield of 5, 6-carboxyl-X-rhodamine is increased. Therefore, the reaction conversion rate and the purification simplicity are obviously improved; 5, 6-carboxyl-X-rhodamine and organic amine are salified, so that the separation cost is greatly reduced, the operation steps are simplified, and the industrial production of carboxyl-X-rhodamine becomes possible; an acid precipitation method is adopted, the 5-carboxyl-X-rhodamine ammonium salt and the 6-carboxyl-X-rhodamine ammonium salt are converted into the 5-carboxyl-X-rhodamine and the 6-carboxyl-X-rhodamine, and the efficiency is high; the whole preparation process has the advantages of simplicity and convenience in operation, low equipment requirement, low cost, high equipment use efficiency and the like, and meets the requirements of industrial production and application.

Compounds and Method for Imaging Cancer

A compound, a method of imaging a cell, and a method of detecting a cancer, an inflammatory disease, or a neoplastic disease are provided. The compound includes a nitroxide derivative of a fluorophore that is activatable upon exposure to free radicals. The method of imaging a cell includes contacting the cell with an activatable nitroxide derivative of a fluorophore. The method of detecting a cancer, an inflammatory disease, or a neoplastic disease includes administering an activatable nitroxide derivative of a fluorophore and then imaging one or more cells contacted with the compound.

CELL-PENETRATING FLUORESCENT DYES WITH SECONDARY ALCOHOL FUNCTIONALITIES

The invention relates to novel cell-penetrating fluorescent dyes with with secondary alcohol functionalities having one of the following general formulae I-III and 4: The invention also relates to the use of these compounds for optical microscopy and imaging techniques.

Hydroxylated Fluorescent Dyes for Live-Cell Labeling: Synthesis, Spectra and Super-Resolution STED

Hydroxylated rhodamines, carbopyronines, silico- and germanorhodamines with absorption maxima in the range of 530–640 nm were prepared and applied in specific labeling of living cells. The direct and high-yielding entry to germa- and silaxanthones tolerates the presence of protected heteroatoms and may be considered for the syntheses of various sila- and germafluoresceins, as well as -rhodols. Application in stimulated emission depletion (STED) fluorescence microscopy revealed a resolution of 50–75 nm in one- and two-color imaging of vimentin-HaloTag fused protein and native tubulin. The established structure–property relationships allow for prediction of the spectral properties and the positions of spirolactone/zwitterion equilibria for the new analogues of rhodamines, carbo-, silico-, and germanorhodamines using simple additive schemes.

METHODS FOR SYNTHESIZING RHODAMINE DYES

Described herein are methods for the regioselective synthesis of substituted rhodamine dyes.

Scalable Regioselective Synthesis of Rhodamine Dyes

A one-step, operationally simple protocol for the synthesis of isomerically pure rhodamine dyes from phthalaldehydic acids is reported. Using a mixture of 2,2,2-trifluoroethanol and water as reaction media allows for clean and efficient formation of vario

Nucleic acid size detection method

The present invention provides methods of determining the size of a particular nucleic acid segment of interest in a sample of nucleic acids through fragmentation of DNA, size fractionation, an optional second fragmentation, and identification using a marker sequence. In particular aspects, an expansion or reduction of tandem repeat sequences can be detected. In further aspects, carriers and individuals afflicted with fragile X syndrome or other diseases associated with tandem repeats can be distinguished from normal individuals.

Substractive single label comparative hybridization

Provided are methods of determining differences between nucleic acids in a test sample and a reference sample. In certain embodiments the methods are used for detecting and mapping chromosomal or genetic abnormalities associated with various diseases or with predisposition to various diseases, or to detecting the phenomena of large scale copy number variants. In particular, provided are advanced methods of performing array-based comparative hybridization that allow reproducibility between samples and enhanced sensitivity by using the same detectable label for both test sample and reference sample nucleic acids. Invention methods are useful for the detection or diagnosis of particular disease conditions such as cancer, and detecting predisposition to cancer based on detection of chromosomal or genetic abnormalities and gene expression level. Invention methods are also useful for the detection or diagnosis of hereditary genetic disorders or predisposition thereto, especially in prenatal samples. Moreover, invention methods are also useful for the detection or diagnosis of de novo genetic aberrations associated with post-natal developmental abnormalities.

Synthesis of 5- and 6-carboxy-x-rhodamines

(Equation Presented) An efficient route is reported to 5- and 6-carboxy-X-rhodamines (compounds 1 and 2) that contain multiple n-propylene or y,y-dimethylpropylene groups bridging terminal nitrogen atoms and the central xanthene core. Gram quantities of these dyes are synthesized from inexpensive starting materials. The isolated products are activated by selective transformation of the carboxylic acid group into N-hydroxysuccinimidyl esters in situ and then conjugated with an amino group of a molecule of interest.