203510-50-3Relevant academic research and scientific papers
Hapten Synthesis for a Monoclonal Antibody Based ELISA for Deltamethrin
Queffelec, Anne-Laurence,Nodet, Patrice,Haelters, Jean-Pierre,Thouvenot, Daniel,Corbel, Bernard
, p. 1670 - 1676 (1998)
An enzyme-linked immunosorbent assay (ELISA) was developed for the insecticide deltamethrin. Two haptens were synthesized: the first one was a derivative of the whole molecule with a spacer arm bound to the aromatic ring; the second one was a derivative of deltamethric acid. Twelve monoclonal antibodies were obtained. A competitive ELISA using monoclonal antibody Del 01 yielded the most sensitive assay. The IC50 value for deltamethrin was estimated to be 0.5 μg mL-1 with a detection limit of 0.08,μg mL-1. Monoclonal antibody Del 01 seems to be deltamethrin specific since 1% cross-reactivity with other pyrethroid analogues was observed.
