20671-66-3

Basic information

• Product Name: 4-METHYLUMBELLIFERYL CAPRYLATE
• Synonyms: OCTANOIC ACID 4-METHYLUMBELLIFERYL ESTER;4-METHYLUMBELLIFERYL CAPRYLATE;4-MU-CAPRYLATE;4-METHYLUMBELLIFERYL
• CAS NO: 20671-66-3
• Molecular Formula: C₁₈H₂₂O₄
• Molecular Weight: 302.36
• Product Categories: substrate
• Mol File: 20671-66-3.mol
• Article Data: 4

Chemical Properties

• Melting Point: 68-69 °C(Solv: ethanol (64-17-5))
• Boiling Point: 441.1 °C at 760 mmHg
• Flash Point: 219.7 °C
• Appearance: white to slightly off-white crystalline powder
• Density: 1.112 g/cm³
• Vapor Pressure: 5.59E-08mmHg at 25°C
• Refractive Index: 1.527
• Storage Temp.: Store at 0°C
• Solubility: Acetonitrile (Slightly), DMSO (Slightly)
• CAS DataBase Reference: 4-METHYLUMBELLIFERYL CAPRYLATE(CAS DataBase Reference)
• NIST Chemistry Reference: 4-METHYLUMBELLIFERYL CAPRYLATE(20671-66-3)
• EPA Substance Registry System: 4-METHYLUMBELLIFERYL CAPRYLATE(20671-66-3)

Safety Data

• Hazardous Substances Data: 20671-66-3(Hazardous Substances Data)

Usage

Uses

4-Methylumbelliferyl Caprylate is a fluorogenic substrate for esterase with C8 activity yielding a blue fluorescent solution upon cleavage. Employed in the identification of Salmonella spp. based on the detection of caprylate esterase.

InChI:InChI=1/C18H22O4/c1-3-4-5-6-7-8-17(19)21-14-9-10-15-13(2)11-18(20)22-16(15)12-14/h9-12H,3-8H2,1-2H3

Upstream product

• 90-33-5 7-hydroxy-4-methyl-chromen-2-one 
• 111-64-8 n-octanoic acid chloride 
• 108-46-3 recorcinol 
• 124-07-2 Octanoic acid 
• 5980-33-6 sodium 4-methylumbelliferonate 

Downstream Products

• 90-33-5 7-hydroxy-4-methyl-chromen-2-one 
• 124-07-2 Octanoic acid 

Relevant articles and documents

Lipase catalysed substituted coumarins with antioxidant activity

Lipase has been employed as a green catalyst to esterify 7- and 6-hydroxy-4-methyl-coumarin with different fatty and acetic acid. The esterified coumarins have been evaluated for antioxidant activities using DPPH and ABTS assays, among them 3a and 5a are known as active antioxidants.

A high-throughput, low-volume enzyme assay on solid support

A high-throughput enzyme assay is described that uses 1 μL or less of enzyme solution for each test Enzyme solutions are deposited by robotic handling in a throughput of over 1000 tests/h on the surface of silica gel plates that have been preimpregnated with fluorogenic substrates. The reaction is quantitated by fluorescence. The method is compatible with water-insoluble substrates (lipases), water-soluble substrates (glycosidases), whole-protein substrates (proteases), and enzyme inhibition measurements. Hydrolytically labile umbelliferyl esters can be used to assay lipases in this format without background hydrolysis. High throughput and reproducibility were tested by fingerprint analysis of lipases and esterases against 37 different fluorogenic ester substrates. A set of eight fluorogenic unbelliferyl esters was selected for optimal activity screening of lipases and esterases on silica gel plates.